Literature DB >> 17021116

Comparison of PCR-based methods for typing Staphylococcus aureus isolates.

Artur Sabat1, Natalia Malachowa, Jacek Miedzobrodzki, Waleria Hryniewicz.   

Abstract

In this study, we compared the potentials of (i) a multiplex PCR-based multilocus variable-number tandem repeat (VNTR) assay; (ii) a triplex PCR coamplifying fragments of spa, coa, and the hypervariable region adjacent to the mecA gene; (iii) restriction profile analysis of the STAR repetitive element; (iv) randomly amplified polymorphic DNA analysis; (v) inter-IS256 PCR; and (vi) rep-MP3 PCR. Multilocus VNTR typing and triplex PCR (coa, spa, and hypervariable region) approaches showed excellent reproducibility and high discriminatory power; however, only multilocus VNTR typing could distinguish all pulsed-field gel electrophoresis and spa types. Multilocus VNTR typing appears to be the most useful PCR-based method for the rapid genotyping of Staphylococcus aureus strains.

Entities:  

Mesh：

Year:  2006        PMID： 17021116      PMCID： PMC1594760          DOI： 10.1128/JCM.00395-06

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  24 in total

1.  Determining confidence intervals when measuring genetic diversity and the discriminatory abilities of typing methods for microorganisms.

Authors:  H Grundmann; S Hori; G Tanner
Journal:  J Clin Microbiol       Date:  2001-11       Impact factor: 5.948

2.  Consensus guidelines for appropriate use and evaluation of microbial epidemiologic typing systems.

Authors:  M. J. Struelens
Journal:  Clin Microbiol Infect       Date:  1996-08       Impact factor: 8.067

3.  Comparison of pulsed-field gel electrophoresis and coagulase gene restriction profile analysis techniques in the molecular typing of Staphylococcus aureus.

Authors:  C S Chiou; H L Wei; L C Yang
Journal:  J Clin Microbiol       Date:  2000-06       Impact factor: 5.948

4.  Typing of methicillin-resistant Staphylococcus aureus in a university hospital setting by using novel software for spa repeat determination and database management.

Authors:  Dag Harmsen; Heike Claus; Wolfgang Witte; Jörg Rothgänger; Hermann Claus; Doris Turnwald; Ulrich Vogel
Journal:  J Clin Microbiol       Date:  2003-12       Impact factor: 5.948

5.  Assessment of resolution and intercenter reproducibility of results of genotyping Staphylococcus aureus by pulsed-field gel electrophoresis of SmaI macrorestriction fragments: a multicenter study.

Authors:  A van Belkum; W van Leeuwen; M E Kaufmann; B Cookson; F Forey; J Etienne; R Goering; F Tenover; C Steward; F O'Brien; W Grubb; P Tassios; N Legakis; A Morvan; N El Solh; R de Ryck; M Struelens; S Salmenlinna; J Vuopio-Varkila; M Kooistra; A Talens; W Witte; H Verbrugh
Journal:  J Clin Microbiol       Date:  1998-06       Impact factor: 5.948

6.  Numerical index of the discriminatory ability of typing systems: an application of Simpson's index of diversity.

Authors:  P R Hunter; M A Gaston
Journal:  J Clin Microbiol       Date:  1988-11       Impact factor: 5.948

7.  Use of an automated multiple-locus, variable-number tandem repeat-based method for rapid and high-throughput genotyping of Staphylococcus aureus isolates.

Authors:  Patrice Francois; Antoine Huyghe; Yvan Charbonnier; Manuela Bento; Sébastien Herzig; Ivan Topolski; Bénédicte Fleury; Daniel Lew; Pierre Vaudaux; Stephan Harbarth; Willem van Leeuwen; Alex van Belkum; Dominique S Blanc; Didier Pittet; Jacques Schrenzel
Journal:  J Clin Microbiol       Date:  2005-07       Impact factor: 5.948

8.  Comparison of multiple-locus variable-number tandem-repeat analysis with pulsed-field gel electrophoresis, spa typing, and multilocus sequence typing for clonal characterization of Staphylococcus aureus isolates.

Authors:  Natalia Malachowa; Artur Sabat; Marek Gniadkowski; Jolanta Krzyszton-Russjan; Joanna Empel; Jacek Miedzobrodzki; Klaudia Kosowska-Shick; Peter C Appelbaum; Waleria Hryniewicz
Journal:  J Clin Microbiol       Date:  2005-07       Impact factor: 5.948

Review 9.  Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing.

Authors:  F C Tenover; R D Arbeit; R V Goering; P A Mickelsen; B E Murray; D H Persing; B Swaminathan
Journal:  J Clin Microbiol       Date:  1995-09       Impact factor: 5.948

10.  Pulsed-field gel electrophoresis typing of oxacillin-resistant Staphylococcus aureus isolates from the United States: establishing a national database.

Authors:  Linda K McDougal; Christine D Steward; George E Killgore; Jasmine M Chaitram; Sigrid K McAllister; Fred C Tenover
Journal:  J Clin Microbiol       Date:  2003-11       Impact factor: 5.948
View more
  17 in total

1.  Rapid identification of methicillin-resistant Staphylococcus aureus transmission in hospitals by use of phage-derived open reading frame typing enhanced by multiplex PCR and reverse line blot assay.

Authors:  Matthew V N O'Sullivan; Fanrong Kong; Vitali Sintchenko; Gwendolyn L Gilbert
Journal:  J Clin Microbiol       Date:  2010-06-02       Impact factor: 5.948

2.  Incidence and characteristics of vancomycin nonsusceptible strains of methicillin-resistant Staphylococcus aureus at Hershey Medical Center.

Authors:  Klaudia Kosowska-Shick; Lois M Ednie; Pamela McGhee; Kathy Smith; Cynthia D Todd; Amanda Wehler; Peter C Appelbaum
Journal:  Antimicrob Agents Chemother       Date:  2008-10-06       Impact factor: 5.191

Review 3.  Small colony variants of Staphylococcus aureus--review.

Authors:  O Melter; B Radojevič
Journal:  Folia Microbiol (Praha)       Date:  2011-01-21       Impact factor: 2.099

Review 4.  Multilocus variable number tandem repeat analysis for Salmonella enterica subspecies.

Authors:  S L Kruy; H van Cuyck; J L Koeck
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2010-12-11       Impact factor: 3.267

5.  Comparison of fingerprinting methods for typing methicillin-resistant Staphylococcus aureus sequence type 398.

Authors:  G Rasschaert; W Vanderhaeghen; I Dewaele; N Janez; X Huijsdens; P Butaye; M Heyndrickx
Journal:  J Clin Microbiol       Date:  2009-08-26       Impact factor: 5.948

6.  Improved multiple-locus variable-number tandem-repeat assay for Staphylococcus aureus genotyping, providing a highly informative technique together with strong phylogenetic value.

Authors:  Christine Pourcel; Katia Hormigos; Lucie Onteniente; Olga Sakwinska; Ruud H Deurenberg; Gilles Vergnaud
Journal:  J Clin Microbiol       Date:  2009-08-26       Impact factor: 5.948

7.  Countrywide molecular survey of methicillin-resistant Staphylococcus aureus strains in Poland.

Authors:  Agnieszka Łuczak-Kadłubowska; Agnieszka Sulikowska; Joanna Empel; Anna Piasecka; Monika Orczykowska; Aleksandra Kozinska; Waleria Hryniewicz
Journal:  J Clin Microbiol       Date:  2008-07-09       Impact factor: 5.948

Review 8.  Methicillin-Resistant Staphylococcus aureus: Molecular Characterization, Evolution, and Epidemiology.

Authors:  Sahreena Lakhundi; Kunyan Zhang
Journal:  Clin Microbiol Rev       Date:  2018-09-12       Impact factor: 26.132

9.  Microfluidic-chip-based multiple-locus variable-number tandem-repeat fingerprinting with new primer sets for methicillin-resistant Staphylococcus aureus.

Authors:  Artur J Sabat; Monika A Chlebowicz; Hajo Grundmann; Jan P Arends; Greetje Kampinga; Nico E L Meessen; Alexander W Friedrich; Jan Maarten van Dijl
Journal:  J Clin Microbiol       Date:  2012-05-09       Impact factor: 5.948

10.  Longitudinal survey of Staphylococcus aureus in cystic fibrosis patients using a multiple-locus variable-number of tandem-repeats analysis method.

Authors:  Hoang Vu-Thien; Katia Hormigos; Gaëlle Corbineau; Brigitte Fauroux; Harriet Corvol; Didier Moissenet; Gilles Vergnaud; Christine Pourcel
Journal:  BMC Microbiol       Date:  2010-01-27       Impact factor: 3.605
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.