An efficient, in vitro cyclic production of shoots from adult trees of Crataeva nurvala Buch. Ham.

An efficient, cyclic, two-step protocol for micropropagation of medicinal tree, Crataeva nurvala has been successfully developed, which can be employed at a commercial scale. Nodal explants from 30-year-old tree when cultured on MS medium supplemented with 2.22 microM BAP produced multiple shoots, which elongated satisfactorily on the same medium. Nodal and leaf explants from in vitro regenerated microshoots too developed shoots, thus making the process recurrent. In 6-month duration, owing to the recurring nature of the protocol, over 5400 shoots could be produced from a single nodal explant from the adult tree. Addition of casein hydrolysate significantly increased the average number of shoots per explant. Maximum number of shoots regenerated on medium supplemented with 100 mg l(-1) casein hydrolysate. Shoots could be rooted on 1/2 MS supplemented with 0.11 and 0.54 microM NAA. Regenerated plantlets were acclimatized and successfully transplanted to soil.