BACKGROUND: A major focus of prostate cancer research has been to identify genes that are deregulated during tumor progression, potentially providing diagnostic markers and therapeutic targets. METHODS: We have employed serial analysis of gene expression (SAGE) and microarray hybridization to identify alterations that occur during malignant transformation in the Transgenic Adenocarcinoma of the Mouse Prostate (TRAMP) model. Many of these alterations were validated by real-time PCR (rtPCR). RESULTS: We identified several hundred mRNAs that were deregulated. Cluster analysis of microarray profiles with samples from various stages of the disease demonstrated that androgen-independent (AI) primary tumors are similar to metastases; 180 transcripts have expression patterns suggesting an involvement in the genesis of late-stage tumors, and our data support a role for phospholipase A2 group IIA in the acquisition of their highly aggressive characteristics. CONCLUSIONS: Our analyses identified well-characterized genes that were previously known to be involved in prostate cancer, validating our study, and also uncovered transcripts that had not previously been implicated in prostate cancer progression. (c) 2006 Wiley-Liss, Inc.
BACKGROUND: A major focus of prostate cancer research has been to identify genes that are deregulated during tumor progression, potentially providing diagnostic markers and therapeutic targets. METHODS: We have employed serial analysis of gene expression (SAGE) and microarray hybridization to identify alterations that occur during malignant transformation in the Transgenic Adenocarcinoma of the Mouse Prostate (TRAMP) model. Many of these alterations were validated by real-time PCR (rtPCR). RESULTS: We identified several hundred mRNAs that were deregulated. Cluster analysis of microarray profiles with samples from various stages of the disease demonstrated that androgen-independent (AI) primary tumors are similar to metastases; 180 transcripts have expression patterns suggesting an involvement in the genesis of late-stage tumors, and our data support a role for phospholipase A2 group IIA in the acquisition of their highly aggressive characteristics. CONCLUSIONS: Our analyses identified well-characterized genes that were previously known to be involved in prostate cancer, validating our study, and also uncovered transcripts that had not previously been implicated in prostate cancer progression. (c) 2006 Wiley-Liss, Inc.
Authors: Leslie Oleksowicz; Yin Liu; R Bruce Bracken; Krishnanath Gaitonde; Barbara Burke; Paul Succop; Linda Levin; Zhongyun Dong; Shan Lu Journal: Prostate Date: 2011-11-29 Impact factor: 4.104
Authors: Zhongyun Dong; Yin Liu; Kieran F Scott; Linda Levin; Krishnanath Gaitonde; R Bruce Bracken; Barbara Burke; Qihui Jim Zhai; Jiang Wang; Leslie Oleksowicz; Shan Lu Journal: Carcinogenesis Date: 2010-09-13 Impact factor: 4.944
Authors: Tammy L Romanuik; Gang Wang; Olena Morozova; Allen Delaney; Marco A Marra; Marianne D Sadar Journal: BMC Med Genomics Date: 2010-09-24 Impact factor: 3.063
Authors: Casey M Turk; Katerina D Fagan-Solis; Kristin E Williams; Joseph M Gozgit; Sallie Smith-Schneider; Sharon A Marconi; Christopher N Otis; Giovanna M Crisi; Douglas L Anderton; Manfred W Kilimann; Kathleen F Arcaro Journal: Cancer Cell Int Date: 2012-05-10 Impact factor: 5.722