| Literature DB >> 17011013 |
Rolf Herrmann1, Martin Heck, Peter Henklein, Christiane Kleuss, Victor Wray, Klaus Peter Hofmann, Oliver P Ernst.
Abstract
In the early steps of visual signal transduction, light-activated rhodopsin (R*) catalyzes GDP/GTP exchange in the heterotrimeric G protein (Galphabetagamma) transducin. We recently reported that the catalytic interaction involves two sequential steps. An initial docking between R* and Gbetagamma leads to conformational changes which make the C-terminus of Galpha (CTalpha) available for binding to R*. Binding of CTalpha by R* then triggers GDP/GTP exchange in the Galpha subunit. To further study this two-step mechanism, we investigated different single amino acid substitutions within CTalpha and discuss the effects of high affinity mutations on nucleotide exchange catalysis.Entities:
Mesh：
Substances：
Year: 2006 PMID： 17011013 DOI： 10.1016/j.visres.2006.07.027
Source DB: PubMed Journal: Vision Res ISSN： 0042-6989 Impact factor: 1.886