Literature DB >> 170098

Protein kinase of bacteriophage T7. 1. Purification.

S H Pai, H Ponta, H J Rahmsdorf, M Hirsch-Kauffmann, P Herrlich, M Schweiger.   

Abstract

A protein kinase, ATP:protein phosphotransferase (EC 2.7.1.37) was detected in Escherichia coli after infection with bacteriophage T7. The enzyme was purified from the ribosomal wash fraction by conventional methods, affinity chromatography on Cibacron blue and on lysozyme coupled to Sepharose, and by cellogel electrophoresis. An approximately 5000-fold purification was achieved.

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Year:  1975        PMID: 170098     DOI: 10.1111/j.1432-1033.1975.tb02163.x

Source DB:  PubMed          Journal:  Eur J Biochem        ISSN: 0014-2956


  2 in total

1.  Bacteriophage T7 protein kinase: Site of inhibitory autophosphorylation, and use of dephosphorylated enzyme for efficient modification of protein in vitro.

Authors:  Swapna Gone; Allen W Nicholson
Journal:  Protein Expr Purif       Date:  2012-08-21       Impact factor: 1.650

2.  Mechanism of Ribonuclease III Catalytic Regulation by Serine Phosphorylation.

Authors:  Swapna Gone; Mercedes Alfonso-Prieto; Samridhdi Paudyal; Allen W Nicholson
Journal:  Sci Rep       Date:  2016-05-06       Impact factor: 4.379

  2 in total

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