Culturing neuron cells on electrode with self-assembly monolayer.

The success of neuronal implantable microsystems relies on the quality of the interface with neuronal cells. Depending on the application, specifically engineered surfaces may either prevent or enhance cell/tissue growth with an appropriate host response. The surface chemistry and topography have major effects on the cell adherence and the interaction between the tissue and devices. We report on a simple technique to precisely explant cortical neurons in a serum-free medium on 2D electrode arrays and investigated the pad size effect on neuron cell culture and immobilization. We produced gold patterns on glass substrates using microfabrication processes. 11-Amino-1-undecanethiol self-assembled monolayer was coated only on the gold surface. Cortical neurons were cultured on the arrays to examine the dependence of neuron growth and cells distribution on pad size. We found that the terminal functional groups of the highly oriented 11-amino-1-undecanethiol thin film are essential for generating cell-adhesive areas for the rat cortical neurons. A 50 microm x 50 microm SAM pad size was found to be suitable for single cortical neuron immobilization, while the larger pads provide excellent neuron coverage. This technology may enable precise and localized neuron stimulation and surveillance for both biological research and medical applications.