| Literature DB >> 17007838 |
Jun-Mei Wang1, Yuan-Shan Zeng, Ran-Yi Liu, Wen-Lin Huang, Yi Xiong, Yan-Hua Wang, Shui-Jun Chen, Yang D Teng.
Abstract
We have constructed a recombinant adenovirus expression vector carrying the human neurotrophin-3 (NT-3) receptor TrkC (tyrosine protein kinase C) gene (rAd-TrkC; 2478 bp) and confirmed the expression of the encoded TrkC in green fluorescent protein (GFP)-murine neural stem cells (NSCs) by reverse transcription polymerase chain reaction (RT-PCR), Western blot analysis, and immunocytochemistry. The activity of the expressed rAd-TrkC was verified in vitro by evaluating dose-related responses of NSCs to NT-3, a TrkC specific ligand. TrkC-GFP-NSCs had a significantly higher percentage of neuronal differentiation when treated with NT-3 relative to the rAd-LacZ control cells (55.2% vs. 29.8%; P<0.05, chi(2) test). Thus, our rAd-TrkC vector can transfect NSCs and produce functional TrkC receptors to promote neuronal differentiation of NSCs.Entities:
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Year: 2006 PMID： 17007838 DOI： 10.1016/j.expneurol.2006.07.028
Source DB: PubMed Journal: Exp Neurol ISSN： 0014-4886 Impact factor: 5.330