AIM: To investigate the frequency of the common NOD2/CARD15 susceptibility variants and two functional polymorphisms of OCTN cation transporter genes in Hungarian pediatric patients with Crohn's disease (CD). METHODS: A cohort of 19 unrelated pediatric and 55 unrelated adult patients with Crohn's disease and 49 healthy controls were studied. Genotyping of the three common CD-associated CARD15 variants (Arg702Trp, Gly908Arg and 1007finsC changes) with the SLC22A4 1672C-->T, and SLC22A5 -207G-->C mutations was performed by direct sequencing of the specific regions of these genes. RESULTS: At least one CARD15 mutation was present in 52.6% of the children and in 34.5% of the adults compared to 14.3% in controls. Surprisingly, strongly different mutation profile was detected in the pediatric versus adult patients. While the G908R and 1007finsC variants were 18.4% and 21.1% in the pediatric group, they were 1.82% and 11.8% in the adults, and were 1.02% and 3.06% in the controls, respectively. The R702W allele was increased approximately two-fold in the adult subjects, while in the pediatric group it was only approximately 64% of the controls (9.09% in the adults, 2.63% in pediatric patients, and 4.08% in the controls). No accumulation of the OCTN variants was observed in any patient group versus the controls. CONCLUSION: The frequency of the NOD2/CARD15 susceptibility variants in the Hungarian pediatric CD population is high and the profile differs from the adult CD patients, whereas the results for SLC22A4 and SLC22A5 mutation screening do not confirm the assumption that the carriage of these genotypes means an obligatory susceptibility to CD.
AIM: To investigate the frequency of the common NOD2/CARD15 susceptibility variants and two functional polymorphisms of OCTN cation transporter genes in Hungarian pediatric patients with Crohn's disease (CD). METHODS: A cohort of 19 unrelated pediatric and 55 unrelated adult patients with Crohn's disease and 49 healthy controls were studied. Genotyping of the three common CD-associated CARD15 variants (Arg702Trp, Gly908Arg and 1007finsC changes) with the SLC22A4 1672C-->T, and SLC22A5 -207G-->C mutations was performed by direct sequencing of the specific regions of these genes. RESULTS: At least one CARD15 mutation was present in 52.6% of the children and in 34.5% of the adults compared to 14.3% in controls. Surprisingly, strongly different mutation profile was detected in the pediatric versus adult patients. While the G908R and 1007finsC variants were 18.4% and 21.1% in the pediatric group, they were 1.82% and 11.8% in the adults, and were 1.02% and 3.06% in the controls, respectively. The R702W allele was increased approximately two-fold in the adult subjects, while in the pediatric group it was only approximately 64% of the controls (9.09% in the adults, 2.63% in pediatric patients, and 4.08% in the controls). No accumulation of the OCTN variants was observed in any patient group versus the controls. CONCLUSION: The frequency of the NOD2/CARD15 susceptibility variants in the Hungarian pediatric CD population is high and the profile differs from the adult CD patients, whereas the results for SLC22A4 and SLC22A5 mutation screening do not confirm the assumption that the carriage of these genotypes means an obligatory susceptibility to CD.
Authors: Bill Newman; Xiangjun Gu; Richard Wintle; David Cescon; Mehrdad Yazdanpanah; Xiangdong Liu; Vanya Peltekova; Mark Van Oene; Christopher I Amos; Katherine A Siminovitch Journal: Gastroenterology Date: 2005-02 Impact factor: 22.682
Authors: Dirk Gründemann; Stephanie Harlfinger; Stefan Golz; Andreas Geerts; Andreas Lazar; Reinhard Berkels; Norma Jung; Andrea Rubbert; Edgar Schömig Journal: Proc Natl Acad Sci U S A Date: 2005-03-28 Impact factor: 11.205
Authors: Peter Laszlo Lakatos; Laszlo Lakatos; Ferenc Szalay; Claudia Willheim-Polli; Christoph Osterreicher; Zsolt Tulassay; Tamas Molnar; Walter Reinisch; Janos Papp; Gyula Mozsik; Peter Ferenci Journal: World J Gastroenterol Date: 2005-03-14 Impact factor: 5.742
Authors: Subra Kugathasan; Nicole Collins; Karen Maresso; Raymond G Hoffmann; Michael Stephens; Steven L Werlin; Colin Rudolph; Ulrich Broeckel Journal: Clin Gastroenterol Hepatol Date: 2004-11 Impact factor: 11.382
Authors: J H Cho; D L Nicolae; L H Gold; C T Fields; M C LaBuda; P M Rohal; M R Pickles; L Qin; Y Fu; J S Mann; B S Kirschner; E W Jabs; J Weber; S B Hanauer; T M Bayless; S R Brant Journal: Proc Natl Acad Sci U S A Date: 1998-06-23 Impact factor: 11.205
Authors: Gilaad G Kaplan; Bo V Pedersen; Roland E Andersson; Bruce E Sands; Joshua Korzenik; Morten Frisch Journal: Gut Date: 2007-05-09 Impact factor: 23.059
Authors: S Cucchiara; A Latiano; O Palmieri; A M Staiano; R D'Incà; G Guariso; G Vieni; V Rutigliano; O Borrelli; M R Valvano; V Annese Journal: World J Gastroenterol Date: 2007-02-28 Impact factor: 5.742