Literature DB >> 17006016

Agrobacterium-mediated transformation of chickpea with alpha-amylase inhibitor gene for insect resistance.

S Ignacimuthu1, S Prakash.   

Abstract

Chickpea is the world's third most important pulse crop and India produces 75% of the world's supply. Chickpea seeds are attacked by Callosobruchus maculatus and C. chinensis which cause extensive damage. The alpha-amylase inhibitor gene isolated from Phaseolus vulgaris seeds was introduced into chickpea cultivar K850 through Agrobacterium-mediated transformation. A total of 288 kanamycin resistant plants were regenerated. Only 0.3% of these were true transformants. Polymerase chain reaction (PCR) analysis and Southern hybridization confirmed the presence of 4.9 kb alpha-amylase inhibitor gene in the transformed plants. Western blot confirmed the presence of alpha-amylase inhibitor protein. The results of bioassay study revealed a significant reduction in the survival rate of bruchid weevil C. maculatus reared on transgenic chickpea seeds. All the transgenic plants exhibited a segregation ratio of 3:1.

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Year:  2006        PMID： 17006016     DOI： 10.1007/bf02704106

Source DB:  PubMed          Journal:  J Biosci        ISSN： 0250-5991            Impact factor:   1.826


  10 in total

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Authors:  P K. Jaiwal; R Kumari; S Ignacimuthu; I Potrykus; C Sautter
Journal:  Plant Sci       Date:  2001-07       Impact factor: 4.729

Review 2.  Insect pest control by copying nature using genetically engineered crops.

Authors:  D Boulter
Journal:  Phytochemistry       Date:  1993-12       Impact factor: 4.072

3.  Protective mechanism of the Mexican bean weevil against high levels of alpha-amylase inhibitor in the common bean.

Authors:  M Ishimoto; M J Chrispeels
Journal:  Plant Physiol       Date:  1996-06       Impact factor: 8.340

4.  Bean alpha-amylase inhibitor 1 in transgenic peas (Pisum sativum) provides complete protection from pea weevil (Bruchus pisorum) under field conditions.

Authors:  R L Morton; H E Schroeder; K S Bateman; M J Chrispeels; E Armstrong; T J Higgins
Journal:  Proc Natl Acad Sci U S A       Date:  2000-04-11       Impact factor: 11.205

5.  An efficient protocol for shoot regeneration and genetic transformation of pigeonpea [ Cajanus cajan (L) Millsp] using leaf explants.

Authors:  S Dayal; M Lavanya; P Devi; K K Sharma
Journal:  Plant Cell Rep       Date:  2003-05-07       Impact factor: 4.570

6.  Bean [alpha]-Amylase Inhibitor Confers Resistance to the Pea Weevil (Bruchus pisorum) in Transgenic Peas (Pisum sativum L.).

Authors:  H. E. Schroeder; S. Gollasch; A. Moore; L. M. Tabe; S. Craig; D. C. Hardie; M. J. Chrispeels; D. Spencer; TJV. Higgins
Journal:  Plant Physiol       Date:  1995-04       Impact factor: 8.340

7.  Stable genetic transformation of Vigna mungo L. Hepper via Agrobacterium tumefaciens.

Authors:  R Saini; P K Jaiwal; S Jaiwal
Journal:  Plant Cell Rep       Date:  2003-03-22       Impact factor: 4.570

8.  Influence of Agrobacterium tumefaciens strain on the production of transgenic peas ( Pisum sativum L.).

Authors:  J E Grant; L M J Thomson; M D Pither-Joyce; T M Dale; P A Cooper
Journal:  Plant Cell Rep       Date:  2003-06-18       Impact factor: 4.570

9.  Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti.

Authors:  G Ditta; S Stanfield; D Corbin; D R Helinski
Journal:  Proc Natl Acad Sci U S A       Date:  1980-12       Impact factor: 11.205

10.  Use of a herbicide or lysine plus threonine for non-antibiotic selection of transgenic chickpea.

Authors:  N Tewari-Singh; J Sen; H Kiesecker; V S Reddy; H-J Jacobsen; S Guha-Mukherjee
Journal:  Plant Cell Rep       Date:  2004-01-29       Impact factor: 4.570
  10 in total
  8 in total

Review 1.  An Update on Genetic Modification of Chickpea for Increased Yield and Stress Tolerance.

Authors:  Manoj Kumar; Mohd Aslam Yusuf; Manisha Nigam; Manoj Kumar
Journal:  Mol Biotechnol       Date:  2018-08       Impact factor: 2.695

2.  Transgenic cowpea (Vigna unguiculata) seeds expressing a bean alpha-amylase inhibitor 1 confer resistance to storage pests, bruchid beetles.

Authors:  Siva Kumar Solleti; Souvika Bakshi; Jubilee Purkayastha; Sanjib Kumar Panda; Lingaraj Sahoo
Journal:  Plant Cell Rep       Date:  2008-09-11       Impact factor: 4.570

3.  Development of a phosphomannose isomerase-based Agrobacterium-mediated transformation system for chickpea (Cicer arietinum L.).

Authors:  Gunvant Patil; Amit Deokar; P K Jain; R J Thengane; R Srinivasan
Journal:  Plant Cell Rep       Date:  2009-08-27       Impact factor: 4.570

4.  Alpha-amylase inhibitor-1 gene from Phaseolus vulgaris expressed in Coffea arabica plants inhibits alpha-amylases from the coffee berry borer pest.

Authors:  Aulus E A D Barbosa; Erika V S Albuquerque; Maria C M Silva; Djair S L Souza; Osmundo B Oliveira-Neto; Arnubio Valencia; Thales L Rocha; Maria F Grossi-de-Sa
Journal:  BMC Biotechnol       Date:  2010-06-17       Impact factor: 2.563

5.  Expression of a Chimeric Gene Encoding Insecticidal Crystal Protein Cry1Aabc of Bacillus thuringiensis in Chickpea (Cicer arietinum L.) Confers Resistance to Gram Pod Borer (Helicoverpa armigera Hubner.).

Authors:  Alok Das; Subhojit Datta; Shallu Thakur; Alok Shukla; Jamal Ansari; G K Sujayanand; Sushil K Chaturvedi; P A Kumar; N P Singh
Journal:  Front Plant Sci       Date:  2017-08-21       Impact factor: 5.753

Review 6.  Viruses and Phytoparasitic Nematodes of Cicer arietinum L.: Biotechnological Approaches in Interaction Studies and for Sustainable Control.

Authors:  Paola Leonetti; Gian Paolo Accotto; Moemen S Hanafy; Vitantonio Pantaleo
Journal:  Front Plant Sci       Date:  2018-03-15       Impact factor: 5.753

7.  Impact of αAI-1 expressed in genetically modified cowpea on Zabrotes subfasciatus (Coleoptera: Chrysomelidae) and its parasitoid, Dinarmus basalis (Hymenoptera: Pteromalidae).

Authors:  Christoph Lüthi; Fernando Alvarez-Alfageme; Jörg Romeis
Journal:  PLoS One       Date:  2013-06-28       Impact factor: 3.240

8.  Symbiotic nitrogen fixation and endophytic bacterial community structure in Bt-transgenic chickpea (Cicer arietinum L).

Authors:  Das Alok; Harika Annapragada; Shilpa Singh; Senthilkumar Murugesan; Narendra Pratap Singh
Journal:  Sci Rep       Date:  2020-03-25       Impact factor: 4.379
  8 in total

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