BACKGROUND: The association between head and neck squamous cell carcinoma (HNSCC) and allelic variants of glutathione S-transferase M1 (GSTM1) and -T1 (GSTT1) is currently controversial. The present study investigates the prevalences of GSTT1 and GSTM1 polymorphism in a cohort of 100 head and neck cancer patients, 100 healthy donors and 200 controls with non-neoplastic head and neck diseases from Italian Lazio Region. METHODS: The patients with benign head and neck pathologies, as well as the healthy donors were matched for age, sex, cigarette smoke (yes/no) and alcohol consumption (yes/no). Molecular definition of GSTT1 and GSTM1 genotype has been performed by means of allele-specific PCR technique. RESULTS: A significant association between head and neck cancer and GSTM1 null genotype was observed both considering benign disease controls (p=0.001, OR=2.613; 95% C.I.=1.48-4.62), and healthy donors (p=0.0003, OR=3.35; 95% C.I. 1.69-6.67) while no significant association was found with GSTT1 null genotype (p>or=0.14). No interactive association was observed when combining the different genotypes of the two polymorphisms. These results were confirmed after correction for daily number of cigarettes and period of tobacco exposure. CONCLUSIONS: The present study confirms a role for genetic alterations of GSTM1 detoxifying enzyme as a risk factor for the development of HNSCC in patients from the Italian Lazio Region, independently of age, sex and other confounding variables.
BACKGROUND: The association between head and neck squamous cell carcinoma (HNSCC) and allelic variants of glutathione S-transferase M1 (GSTM1) and -T1 (GSTT1) is currently controversial. The present study investigates the prevalences of GSTT1 and GSTM1 polymorphism in a cohort of 100 head and neck cancerpatients, 100 healthy donors and 200 controls with non-neoplastic head and neck diseases from Italian Lazio Region. METHODS: The patients with benign head and neck pathologies, as well as the healthy donors were matched for age, sex, cigarette smoke (yes/no) and alcohol consumption (yes/no). Molecular definition of GSTT1 and GSTM1 genotype has been performed by means of allele-specific PCR technique. RESULTS: A significant association between head and neck cancer and GSTM1 null genotype was observed both considering benign disease controls (p=0.001, OR=2.613; 95% C.I.=1.48-4.62), and healthy donors (p=0.0003, OR=3.35; 95% C.I. 1.69-6.67) while no significant association was found with GSTT1 null genotype (p>or=0.14). No interactive association was observed when combining the different genotypes of the two polymorphisms. These results were confirmed after correction for daily number of cigarettes and period of tobacco exposure. CONCLUSIONS: The present study confirms a role for genetic alterations of GSTM1 detoxifying enzyme as a risk factor for the development of HNSCC in patients from the Italian Lazio Region, independently of age, sex and other confounding variables.
Authors: Gustavo Jacob Lourenço; Erika Furquim Soledade Neves Silva; José Augusto Rinck-Junior; Carlos Takahiro Chone; Carmen Silvia Passos Lima Journal: Tumour Biol Date: 2011-08-26
Authors: G Cadoni; S Boccia; L Petrelli; P Di Giannantonio; D Arzani; A Giorgio; E De Feo; M Pandolfini; P Gallì; G Paludetti; G Ricciardi Journal: Acta Otorhinolaryngol Ital Date: 2012-02 Impact factor: 2.124
Authors: Leonor Varela-Lema; Emanuela Taioli; Alberto Ruano-Ravina; Juan M Barros-Dios; Devasena Anantharaman; Simone Benhamou; Stefania Boccia; Rajani A Bhisey; Gabriella Cadoni; Ettore Capoluongo; Chien-Jen Chen; William Foulkes; Eny Maria Goloni-Bertollo; Ana Hatagima; Richard B Hayes; Takahiko Katoh; Sergio Koifman; Phillip Lazarus; Johannes J Manni; Manoj Mahimkar; Shunji Morita; Jong Park; Kwang-Kyun Park; Erika Cristina Pavarino Bertelli; Enilze Maria de Souza Fonseca Ribeiro; Bidyut Roy; Margaret R Spitz; Richard C Strange; Qingyi Wei; Camille C Ragin Journal: Genet Med Date: 2008-06 Impact factor: 8.822