Literature DB >> 17002334

Monitoring DPA release from a single germinating Bacillus subtilis endospore via surface-enhanced Raman scattering microscopy.

David D Evanoff1, John Heckel, Thomas P Caldwell, Kenneth A Christensen, George Chumanov.   

Abstract

A method for monitoring DPA release from a single germinating Bacillus subtilis endospore is reported. High S/N ratio SERS spectra were obtained with excitation power 3 mW at 647.1 nm and 1 min spectral collection times. The method is proof-of-principle for the SERS detection limit at the single spore level. This represents a 100- to 1000-fold improvement over previously reported detection limits for SERS-based measurements of DPA in endospores.

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Year:  2006        PMID: 17002334     DOI: 10.1021/ja0642717

Source DB:  PubMed          Journal:  J Am Chem Soc        ISSN: 0002-7863            Impact factor:   15.419


  6 in total

Review 1.  Surface-enhanced Raman scattering biomedical applications of plasmonic colloidal particles.

Authors:  Sara Abalde-Cela; Paula Aldeanueva-Potel; Cintia Mateo-Mateo; Laura Rodríguez-Lorenzo; Ramón A Alvarez-Puebla; Luis M Liz-Marzán
Journal:  J R Soc Interface       Date:  2010-05-12       Impact factor: 4.118

2.  Factors affecting variability in time between addition of nutrient germinants and rapid dipicolinic acid release during germination of spores of Bacillus species.

Authors:  Pengfei Zhang; Will Garner; Xuan Yi; Ji Yu; Yong-qing Li; Peter Setlow
Journal:  J Bacteriol       Date:  2010-05-14       Impact factor: 3.490

3.  Characterization of bacterial spore germination using phase-contrast and fluorescence microscopy, Raman spectroscopy and optical tweezers.

Authors:  Lingbo Kong; Pengfei Zhang; Guiwen Wang; Jing Yu; Peter Setlow; Yong-qing Li
Journal:  Nat Protoc       Date:  2011-04-21       Impact factor: 13.491

4.  Surface-enhanced Raman scattering (SERS) cytometry.

Authors:  John P Nolan; David S Sebba
Journal:  Methods Cell Biol       Date:  2011       Impact factor: 1.441

5.  Elastic and inelastic light scattering from single bacterial spores in an optical trap allows the monitoring of spore germination dynamics.

Authors:  Lixin Peng; De Chen; Peter Setlow; Yong-qing Li
Journal:  Anal Chem       Date:  2009-05-15       Impact factor: 6.986

Review 6.  Learning from Nature: Bacterial Spores as a Target for Current Technologies in Medicine (Review).

Authors:  B G Andryukov; A A Karpenko; I N Lyapun
Journal:  Sovrem Tekhnologii Med       Date:  2020-06-28
  6 in total

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