Literature DB >> 17001500

Construction and characterization of a novel maltose inducible expression vector in Bacillus subtilis.

Yang Ming-Ming1, Zhang Wei-Wei, Zhang Xi-Feng, Cen Pei-Lin.   

Abstract

A maltose-inducible expression vector in Bacillus subtilis has been developed and characterized. The vector permitted beta-galactosidase expression at a high level (maximum activity, 8.16 U/ml) when induced and its expression was markedly repressed by glucose. Using this vector, we successfully expressed the other two genes, bioA and vgb. This thus provided a potential expression system for cloned genes in B. subtilis.

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Year:  2006        PMID： 17001500     DOI： 10.1007/s10529-006-9146-z

Source DB:  PubMed          Journal:  Biotechnol Lett        ISSN： 0141-5492            Impact factor:   2.461


  13 in total

1.  Expression of plectasin in Bacillus subtilis using SUMO technology by a maltose-inducible vector.

Authors:  Licong Zhang; Xiaodan Li; Dandan Wei; Jue Wang; Anshan Shan; Zhongyu Li
Journal:  J Ind Microbiol Biotechnol       Date:  2015-08-25       Impact factor: 3.346

2.  Production of N-acetyl-D-neuraminic acid by use of an efficient spore surface display system.

Authors:  Xiaoman Xu; Chao Gao; Xifeng Zhang; Bin Che; Cuiqing Ma; Jianhua Qiu; Fei Tao; Ping Xu
Journal:  Appl Environ Microbiol       Date:  2011-03-25       Impact factor: 4.792

3.  Electroporation is a feasible method to introduce circularized or linearized DNA into B. subtilis chromosome.

Authors:  Ming-Ming Yang; Wei-Wei Zhang; Xiao-Ting Bai; Heng-Xin Li; Pei-Lin Cen
Journal:  Mol Biol Rep       Date:  2009-08-11       Impact factor: 2.316

4.  Surface display of the thermophilic lipase Tm1350 on the spore of Bacillus subtilis by the CotB anchor protein.

Authors:  Huayou Chen; Rui Tian; Zhong Ni; Qing Zhang; Tianxi Zhang; Zhi Chen; Keping Chen; Shengli Yang
Journal:  Extremophiles       Date:  2015-05-31       Impact factor: 2.395

5.  Assay and characterization of an osmolarity inducible promoter newly isolated from Bacillus subtilis.

Authors:  Wei-Wei Zhang; Qiu-Rong Gao; Ming-Ming Yang; Hui Liu; Dun Wang
Journal:  Mol Biol Rep       Date:  2012-02-11       Impact factor: 2.316

6.  Development and application of a novel signal peptide probe vector with PGA as reporter in Bacillus subtilis WB700: twenty-four tat pathway signal peptides from Bacillus subtilis were monitored.

Authors:  Fa-Ming Zhu; Sheng-Yue Ji; Wei-Wei Zhang; Wang Li; Bin-Yun Cao; Ming-Ming Yang
Journal:  Mol Biotechnol       Date:  2008-02-06       Impact factor: 2.695

7.  Development of a Bacillus subtilis expression system using the improved Pglv promoter.

Authors:  Yang M Ming; Zhang W Wei; Chen Y Lin; Gong Y Sheng
Journal:  Microb Cell Fact       Date:  2010-07-10       Impact factor: 5.328

8.  Regulation of mtl operon promoter of Bacillus subtilis: requirements of its use in expression vectors.

Authors:  Kambiz Morabbi Heravi; Marian Wenzel; Josef Altenbuchner
Journal:  Microb Cell Fact       Date:  2011-10-20       Impact factor: 5.328

9.  Improved production of sublancin via introduction of three characteristic promoters into operon clusters responsible for this novel distinct glycopeptide biosynthesis.

Authors:  Shengyue Ji; Weili Li; Abdul Rasheed Baloch; Meng Wang; Binyun Cao
Journal:  Microb Cell Fact       Date:  2015-02-12       Impact factor: 5.328

10.  Generation of an artificial double promoter for protein expression in Bacillus subtilis through a promoter trap system.

Authors:  Mingming Yang; Weiwei Zhang; Shengyue Ji; Pinghua Cao; Yulin Chen; Xin Zhao
Journal:  PLoS One       Date:  2013-02-08       Impact factor: 3.240
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