| Literature DB >> 16997384 |
Alexandre T Leal1, Adriana Seixas, Paula C Pohl, Carlos A S Ferreira, Carlos Logullo, Pedro L Oliveira, Sandra E Farias, Carlos Termignoni, Itabajara da Silva Vaz, Aoi Masuda.
Abstract
Boophilus Yolk pro-Cathepsin (BYC) is an aspartic proteinase found in Boophilus microplus eggs that is involved in the embryogenesis and has been tested as antigen to compose an anti-tick vaccine. The vaccine potential of a recombinant BYC expressed in Escherichia coli (rBYC) was investigated. rBYC was purified and used to immunize Hereford cattle. The sera of bovines immunized with rBYC recognized the native BYC with a titer ranging from 125 to 4000. Furthermore, immunized bovines challenged with 20,000 larvae presented an overall protection of 25.24%. The partial protection obtained against B. microplus infestation with the recombinant protein immunization was similar to the already described for native BYC immunization.Entities:
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Year: 2006 PMID： 16997384 DOI： 10.1016/j.vetimm.2006.08.011
Source DB: PubMed Journal: Vet Immunol Immunopathol ISSN： 0165-2427 Impact factor: 2.046