| Literature DB >> 1699307 |
T Iwadare1, E Harada, S Yoshino, T Arai.
Abstract
Development of a resin-dissolving solution for use at low alkali concentrations is described. Crown ether dissolved in dimethyl-sulfoxide produces a superbasic alkoxide anion. A five minute treatment resulted in complete resin removal from kidney biopsy specimens embedded in Epon 812. Specimens were well stained by Loeffler's methylene blue. Periodic acid-methenamine silver and Giemsa stains yielded good results. Application of PAS reaction and subsequent hematoxylin counterstaining was practicable for diagnosis.Entities:
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Year: 1990 PMID： 1699307 DOI： 10.3109/10520299009108071
Source DB: PubMed Journal: Stain Technol ISSN： 0038-9153