OBJECTIVES: To evaluate the effect of ethanolic neem leaf extract (ENLE) on cell proliferation, differentiation and apoptosis associated proteins during 7,12-dimethylbenz[a]anthracene (DMBA)-induced hamster buccal pouch (HBP) carcinogenesis. DESIGN AND METHODS: Hamsters were divided into four groups. The right buccal pouches of animals in group 1 were painted with 0.5% DMBA three times a week. Animals in group 2 painted with DMBA as in group 1, received in addition, intragastric administration of ENLE (200 mg/kg bw) on days alternate to DMBA application. Group 3 animals were given ENLE (200 mg/kg bw) alone. Animals in group 4 served as control. All the animals were sacrificed after an experimental period of 14 weeks. The expression of proliferating cell nuclear antigen (PCNA), cytokeratin, Bcl-2 and p53 in the buccal pouch tissues were investigated using immunohistochemical staining. In addition, the expression of p53 was confirmed by Western blot analysis. RESULTS: Topical application of DMBA for 14 weeks induced buccal pouch carcinomas associated with increased expression of PCNA, mutant p53 and Bcl-2 and decreased expression of cytokeratin. Administration of ENLE significantly inhibited the development of HBP carcinomas as revealed by decreased expression of PCNA, mutant p53 and Bcl-2 and overexpression of cytokeratin. CONCLUSION: These findings suggest that ENLE exerts its anticancer properties by inhibiting cell proliferation and inducing differentiation and apoptosis.
OBJECTIVES: To evaluate the effect of ethanolic neem leaf extract (ENLE) on cell proliferation, differentiation and apoptosis associated proteins during 7,12-dimethylbenz[a]anthracene (DMBA)-induced hamster buccal pouch (HBP) carcinogenesis. DESIGN AND METHODS: Hamsters were divided into four groups. The right buccal pouches of animals in group 1 were painted with 0.5% DMBA three times a week. Animals in group 2 painted with DMBA as in group 1, received in addition, intragastric administration of ENLE (200 mg/kg bw) on days alternate to DMBA application. Group 3 animals were given ENLE (200 mg/kg bw) alone. Animals in group 4 served as control. All the animals were sacrificed after an experimental period of 14 weeks. The expression of proliferating cell nuclear antigen (PCNA), cytokeratin, Bcl-2 and p53 in the buccal pouch tissues were investigated using immunohistochemical staining. In addition, the expression of p53 was confirmed by Western blot analysis. RESULTS: Topical application of DMBA for 14 weeks induced buccal pouch carcinomas associated with increased expression of PCNA, mutant p53 and Bcl-2 and decreased expression of cytokeratin. Administration of ENLE significantly inhibited the development of HBP carcinomas as revealed by decreased expression of PCNA, mutant p53 and Bcl-2 and overexpression of cytokeratin. CONCLUSION: These findings suggest that ENLE exerts its anticancer properties by inhibiting cell proliferation and inducing differentiation and apoptosis.
Authors: Swagata Karkare; Rishi Raj Chhipa; Jane Anderson; Xiaona Liu; Heather Henry; Anjelika Gasilina; Nicholas Nassar; Jayeeta Ghosh; Jason P Clark; Ashish Kumar; Giovanni M Pauletti; Pradip K Ghosh; Biplab Dasgupta Journal: Clin Cancer Res Date: 2013-10-29 Impact factor: 12.531
Authors: G Harish Kumar; R Vidya Priyadarsini; G Vinothini; P Vidjaya Letchoumy; S Nagini Journal: Invest New Drugs Date: 2009-05-21 Impact factor: 3.850
Authors: Saswati Mahapatra; R Jeffrey Karnes; Michael W Holmes; Charles Y F Young; John C Cheville; Manish Kohli; Eric W Klee; Donald J Tindall; Krishna Vanaja Donkena Journal: AAPS J Date: 2011-05-11 Impact factor: 4.009