Guanosine 5'-[gamma-thio]triphosphate-mediated activation of cytosol phospholipase C caused lysosomal destabilization.

Lysosomal disintegration is critical for the organelle functions and cellular viability. In this study, we established that guanosine 5'-[gamma-thio]triphosphate (GTP-gamma-S)-activated cytosol of rat hepatocytes could increase lysosomal permeability to both potassium ions and protons and osmotically destabilize the lysosomes via K(+)/H(+) exchange. These results were obtained through measurements of lysosomal beta-hexosaminidase-free activity, membrane potential and intralysosomal pH. Assays of phospholipase C (PLC) activity show that cytosolic PLC was activated upon addition of GTP-gamma-S to the cytosol. The effects of cytosol on the lysosomes could be abolished by D609, an inhibitor of PLC, but not by the inhibitors of phospholipase A(2). The cytosol-treated lysosomes disintegrated markedly in hypotonic sucrose medium, reflecting that the lysosomal osmotic sensitivity increased. Microscopic observations showed that the lysosomes became more swollen in hypotonic sucrose medium. This indicates that the cytosol treatment induced osmotic shock to the lysosomes and an influx of water into the organelle.