Literature DB >> 16988429

Construction of plasmids for RNA interference and in vitro transcription of double-stranded RNA.

Lisa Timmons1.   

Abstract

Double-stranded RNA (dsRNA)-induced gene silencing in Caenorhabditis elegans involves the manufacture and delivery of defined sequences of dsRNA to the organism, followed by a careful monitoring for loss-of-function phenocopies in treated animals. In this chapter, we describe how to generate DNAs that can be used as templates for transcription of dsRNA.

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Year:  2006        PMID： 16988429     DOI： 10.1385/1-59745-151-7:109

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  5 in total

1.  Role of oxygen consumption in hypoxia protection by translation factor depletion.

Authors:  Barbara Scott; Chun-Ling Sun; Xianrong Mao; Cong Yu; Bhupinder P S Vohra; Jeffrey Milbrandt; C Michael Crowder
Journal:  J Exp Biol       Date:  2013-03-26       Impact factor: 3.312

2.  Exogenous application of double-stranded RNA molecules from TMV p126 and CP genes confers resistance against TMV in tobacco.

Authors:  Naga Charan Konakalla; Athanasios Kaldis; Margarita Berbati; Hema Masarapu; Andreas E Voloudakis
Journal:  Planta       Date:  2016-07-25       Impact factor: 4.116

3.  Loss of NDG-4 extends lifespan and stress resistance in Caenorhabditis elegans.

Authors:  Jeanette Brejning; Steffen Nørgaard; Lone Schøler; Tine H Morthorst; Helle Jakobsen; Gordon J Lithgow; Louise T Jensen; Anders Olsen
Journal:  Aging Cell       Date:  2013-11-28       Impact factor: 9.304

4.  NDK-1, the homolog of NM23-H1/H2 regulates cell migration and apoptotic engulfment in C. elegans.

Authors:  Luca Fancsalszky; Eszter Monostori; Zsolt Farkas; Ehsan Pourkarimi; Neda Masoudi; Balázs Hargitai; Maja Herak Bosnar; Martina Deželjin; Annamária Zsákai; Tibor Vellai; Anil Mehta; Krisztina Takács-Vellai
Journal:  PLoS One       Date:  2014-03-21       Impact factor: 3.240

5.  The SDHB Arg230His mutation causing familial paraganglioma alters glycolysis in a new Caenorhabditis elegans model.

Authors:  Éva Saskői; Zoltán Hujber; Gábor Nyírő; István Likó; Barbara Mátyási; Gábor Petővári; Katalin Mészáros; Attila L Kovács; László Patthy; Shreyas Supekar; Hao Fan; Gergely Sváb; László Tretter; Arunabh Sarkar; Aamir Nazir; Anna Sebestyén; Attila Patócs; Anil Mehta; Krisztina Takács-Vellai
Journal:  Dis Model Mech       Date:  2020-10-15       Impact factor: 5.758
  5 in total

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