| Literature DB >> 16988350 |
Vinitha Cardoza1, C Neal Stewart.
Abstract
High-frequency Agrobacterium tumefaciens-mediated transformation can be obtained in canola by optimizing the preconditioning time of the explant and cocultivation time with A. tumefaciens. A preconditioning time of 72 h and cocultivation of 48 h synergistically increase the transformation efficiency to 25%. In addition, the recovery of transgenic plants can be facilitated by overcoming hyperhydration, which increases the rooting frequency to 100%.Entities:
Mesh:
Year: 2006 PMID: 16988350 DOI: 10.1385/1-59745-130-4:257
Source DB: PubMed Journal: Methods Mol Biol ISSN: 1064-3745