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Literature DB >> 1698189

Reduction of background labeling in colloidal gold-enzyme reactions.

S Squarzoni¹, N Zini, F Marinelli, N M Maraldi.

Abstract

Most methods which are used to prevent a specific colloidal gold (c.g.) binding in cytochemical reactions are based on the addition of macromolecules to the incubation medium. A new method based on the effects of glow discharge (GD) plasma is reported.

Entities: Disease

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Year: 1990 PMID： 1698189 DOI： 10.1007/bf00266631

Source DB: PubMed Journal: Histochemistry ISSN： 0301-5564

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References

10 in total

1. Phospholipase C digestion induces the removal of nuclear RNA: a cytochemical quantitative study.

Authors: N Zini; N M Maraldi; A M Martelli; A Antonucci; P Santi; G Mazzotti; R Rizzoli; F A Manzoli
Journal: Histochem J Date: 1989-08

2. Prevention of non-specific interactions of gold-labeled reagents on tissue sections.

Authors: J Roth; D J Taatjes; M J Warhol
Journal: Histochemistry Date: 1989

3. Cytochemical localization of DNA loop attachment sites to the nuclear lamina and to the inner nuclear matrix.

Authors: N Zini; G Mazzotti; P Santi; R Rizzoli; A Galanzi; R Rana; N M Maraldi
Journal: Histochemistry Date: 1989

4. Adsorption of horseradish peroxidase, ovomucoid and anti-immunoglobulin to colloidal gold for the indirect detection of concanavalin A, wheat germ agglutinin and goat anti-human immunoglobulin G on cell surfaces at the electron microscopic level: a new method, theory and application.

Authors: W D Geoghegan; G A Ackerman
Journal: J Histochem Cytochem Date: 1977-11 Impact factor: 2.479

5. Ultrastructure of cel organelles by scanning electron microscopy of thick sections surface-etched by an oxygen plasma.

Authors: W J Humphreys; W G Henk
Journal: J Microsc Date: 1979-07 Impact factor: 1.758

Reduction of background labeling in colloidal gold-enzyme reactions.

1. Phospholipase C digestion induces the removal of nuclear RNA: a cytochemical quantitative study.

2. Prevention of non-specific interactions of gold-labeled reagents on tissue sections.

3. Cytochemical localization of DNA loop attachment sites to the nuclear lamina and to the inner nuclear matrix.

4. Adsorption of horseradish peroxidase, ovomucoid and anti-immunoglobulin to colloidal gold for the indirect detection of concanavalin A, wheat germ agglutinin and goat anti-human immunoglobulin G on cell surfaces at the electron microscopic level: a new method, theory and application.

5. Ultrastructure of cel organelles by scanning electron microscopy of thick sections surface-etched by an oxygen plasma.

6. A procedure for the isolation of enzymically active rat-liver nuclei.

7. Non-specific binding of protein-stabilized gold sols as a source of error in immunocytochemistry.

8. Ultrastructural localization of nuclei acids by the use of enzyme-gold complexes.

9. Ultrastructural comparison of ion beam and radiofrequency plasma etching effects on biological tissue sections.

10. Perinuclear dense bodies: characterization as DNA-containing structures using enzyme-linked gold granules.