| Literature DB >> 1698153 |
K K Hartmann1, V Papa, E J Brown, U Doerries, S M Rosenthal, I D Goldfine.
Abstract
A simple method was developed to isolate low abundance hormone receptor poly(A)+ RNA from cells in tissue culture. Adherent cells in tissue culture plates were directly released with proteinase K and solubilized in SDS. Oligo(dT) cellulose was directly added to the lysate to obtain poly(A)+ RNA. Yields and purity of the poly(A)+ RNA were comparable to other more lengthy methods. IGF-I receptor and insulin receptor mRNA could be detected on Northern blot without any degradation.Entities:
Mesh：
Substances：
Year: 1990 PMID： 1698153 DOI： 10.1210/endo-127-4-2038
Source DB: PubMed Journal: Endocrinology ISSN： 0013-7227 Impact factor: 4.736