OBJECTIVE: To study the effects of different fractions of Modified Zhisousan (MZ, MZC, MZS) on the contents of nitric oxide (NO), endothelin-1 (ET-1) and eosinophilia (EOS) in the allergic asthma guinea pigs and observe the pathology changes of lung tissue. METHODS: The number of EOS in the blood and bronchialveolar lavage fluid (BALF) was counted by Wright staining. The contents of ET-1 and No in serum and BALF were analyzed by RIA and nitric acid reductase method. The guinea pig lungs were observed under the optical and electron microscope. RESULTS: The number of EOS and the contents of ET-1 and NO in model group were higher than those in control group (P < 0.01). The pathological changes of lung were obvious. The number of EOS and the contents of ET-1 and NO were descended remarkably (P < 0.05 or P < 0.01) and the pathological changes in the lung tissue were lightened obviously in MZ and MZC groups, but MZS group had no such effects. CONCLUSION: MZC is the effective part of MZ and the anti-asthmatic mechanisms ware related to its significant reduction in contents of ET-1, NO, EOS and the damage of lung tissue possibly.
OBJECTIVE: To study the effects of different fractions of Modified Zhisousan (MZ, MZC, MZS) on the contents of nitric oxide (NO), endothelin-1 (ET-1) and eosinophilia (EOS) in the allergic asthmaguinea pigs and observe the pathology changes of lung tissue. METHODS: The number of EOS in the blood and bronchialveolar lavage fluid (BALF) was counted by Wright staining. The contents of ET-1 and No in serum and BALF were analyzed by RIA and nitric acid reductase method. The guinea pig lungs were observed under the optical and electron microscope. RESULTS: The number of EOS and the contents of ET-1 and NO in model group were higher than those in control group (P < 0.01). The pathological changes of lung were obvious. The number of EOS and the contents of ET-1 and NO were descended remarkably (P < 0.05 or P < 0.01) and the pathological changes in the lung tissue were lightened obviously in MZ and MZC groups, but MZS group had no such effects. CONCLUSION:MZC is the effective part of MZ and the anti-asthmatic mechanisms ware related to its significant reduction in contents of ET-1, NO, EOS and the damage of lung tissue possibly.
Authors: Gao Zhen; Wang Jing; Jing Jing; Dan Xu; Li Zheng; Fengsen Li Journal: Evid Based Complement Alternat Med Date: 2018-07-18 Impact factor: 2.629