Literature DB >> 16979134

Construction of a deletion strain and expression vector for the Escherichia coli NADH:ubiquinone oxidoreductase (Complex I).

Bilal Amarneh1, Jessica De Leon-Rangel, Steven B Vik.   

Abstract

Complex I of Escherichia coli is encoded by 13 consecutive genes, called the nuo operon. A chromosomal deletion of all nuo genes has been achieved by homologous recombination. A vector that encodes all of the nuo genes has been constructed, and it expresses a functional enzyme.

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Year:  2006        PMID： 16979134     DOI： 10.1016/j.bbabio.2006.08.003

Source DB:  PubMed          Journal:  Biochim Biophys Acta        ISSN： 0006-3002


  9 in total

1.  Critical roles of subunit NuoH (ND1) in the assembly of peripheral subunits with the membrane domain of Escherichia coli NDH-1.

Authors:  Prem Kumar Sinha; Jesus Torres-Bacete; Eiko Nakamaru-Ogiso; Norma Castro-Guerrero; Akemi Matsuno-Yagi; Takao Yagi
Journal:  J Biol Chem       Date:  2009-02-03       Impact factor: 5.157

2.  Probing the proton channels in subunit N of Complex I from Escherichia coli through intra-subunit cross-linking.

Authors:  Ablat Tursun; Shaotong Zhu; Steven B Vik
Journal:  Biochim Biophys Acta       Date:  2016-09-12

3.  Constraining the Lateral Helix of Respiratory Complex I by Cross-linking Does Not Impair Enzyme Activity or Proton Translocation.

Authors:  Shaotong Zhu; Steven B Vik
Journal:  J Biol Chem       Date:  2015-07-01       Impact factor: 5.157

4.  Analysis of the assembly pathway for membrane subunits of Complex I reveals that subunit L (ND5) can assemble last in E. coli.

Authors:  Fang Zhang; Steven B Vik
Journal:  BBA Adv       Date:  2021-10-17

5.  Loss of Complex I activity in the Escherichia coli enzyme results from truncating the C-terminus of subunit K, but not from cross-linking it to subunits N or L.

Authors:  Shaotong Zhu; Alejandra Canales; Mai Bedair; Steven B Vik
Journal:  J Bioenerg Biomembr       Date:  2016-03-01       Impact factor: 2.945

6.  Features of subunit NuoM (ND4) in Escherichia coli NDH-1: TOPOLOGY AND IMPLICATION OF CONSERVED GLU144 FOR COUPLING SITE 1.

Authors:  Jesus Torres-Bacete; Prem Kumar Sinha; Norma Castro-Guerrero; Akemi Matsuno-Yagi; Takao Yagi
Journal:  J Biol Chem       Date:  2009-10-08       Impact factor: 5.157

7.  Mutagenesis of the L, M, and N subunits of Complex I from Escherichia coli indicates a common role in function.

Authors:  Jose Michel; Jessica DeLeon-Rangel; Shaotong Zhu; Kalie Van Ree; Steven B Vik
Journal:  PLoS One       Date:  2011-02-28       Impact factor: 3.240

8.  Human clinical mutations in mitochondrially encoded subunits of Complex I can be successfully modeled in E. coli.

Authors:  Fang Zhang; Quynh-Chi L Dang; Steven B Vik
Journal:  Mitochondrion       Date:  2022-03-17       Impact factor: 4.534

9.  Comparative genomics of Wolbachia and the bacterial species concept.

Authors:  Kirsten Maren Ellegaard; Lisa Klasson; Kristina Näslund; Kostas Bourtzis; Siv G E Andersson
Journal:  PLoS Genet       Date:  2013-04-04       Impact factor: 5.917
  9 in total

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