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Literature DB >> 1697150

Production and epitope analysis of monoclonal antibodies against a Rhizobium leguminosarum biovar trifolii strain.

Abstract

Heat-treated cells of Rhizobium leguminosarum biovar trifolii strain 162X95 were used to produce monoclonal antibodies (MAbs). The fusion produced three cross-reactive MAbs and eight MAbs specific for the immunizing strain and a group of five other R. trifolii strains from the same geographic region where 162X95 was isolated (California). Seven MAbs were analyzed by competitive enzyme-linked immunosorbent assay to determine the number of different epitopes detectable on strain 162X95. The results indicated that six MAbs reacted with the same or overlapping epitopes, and the seventh MAb gave inconclusive results.

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Year: 1990 PMID： 1697150 PMCID： PMC184596 DOI： 10.1128/aem.56.7.2262-2264.1990

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

13 in total

1. Production and Use of Monoclonal Antibodies for Identification of Strains of Rhizobium trifolii.

Authors: S F Wright; J G Foster; O L Bennett
Journal: Appl Environ Microbiol Date: 1986-07 Impact factor: 4.792

2. Solution-phase RIA and solid-phase EIA using avidin-biotin systems for analysis of monoclonal antibody epitopes and affinity constants.

Authors: J E Shively; C Wagener; B R Clark
Journal: Methods Enzymol Date: 1986 Impact factor: 1.600

3. A rapid and simple ELISA for the determination of duplicate monoclonal antibodies during epitope analysis of antigens and its application to the study of C1(-)-INH.

Authors: J Alsenz; M Loos
Journal: J Immunol Methods Date: 1988-04-22 Impact factor: 2.303

Review 4. The nature of an antigenic determinant.

Authors: E A Kabat
Journal: J Immunol Date: 1966-07 Impact factor: 5.422

5. Immunoaffinity isolation of membrane antigens with biotinylated monoclonal antibodies and streptavidin-agarose.

Authors: T V Updyke; G L Nicolson
Journal: Methods Enzymol Date: 1986 Impact factor: 1.600

6. A convenient enzyme-linked immunosorbent assay for testing whether monoclonal antibodies recognize the same antigenic site. Application to hybridomas specific for the beta 2-subunit of Escherichia coli tryptophan synthase.

Authors: B Friguet; L Djavadi-Ohaniance; J Pages; A Bussard; M Goldberg
Journal: J Immunol Methods Date: 1983-06-10 Impact factor: 2.303

7. "Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A.

Authors: W N Burnette
Journal: Anal Biochem Date: 1981-04 Impact factor: 3.365

2. Application of Recombinant Human scFv Antibody as a Powerful Tool to Monitor Nitrogen Fixing Biofertilizer in Rice and Legume.

Authors: K K Khaing; K Rangnoi; H Michlits; N Boonkerd; N Teaumroong; P Tittabutr; M Yamabhai
Journal: Microbiol Spectr Date: 2021-12-15

2 in total

Production and epitope analysis of monoclonal antibodies against a Rhizobium leguminosarum biovar trifolii strain.

1. Production and Use of Monoclonal Antibodies for Identification of Strains of Rhizobium trifolii.

2. Solution-phase RIA and solid-phase EIA using avidin-biotin systems for analysis of monoclonal antibody epitopes and affinity constants.

3. A rapid and simple ELISA for the determination of duplicate monoclonal antibodies during epitope analysis of antigens and its application to the study of C1(-)-INH.

Review 4. The nature of an antigenic determinant.

5. Immunoaffinity isolation of membrane antigens with biotinylated monoclonal antibodies and streptavidin-agarose.

6. A convenient enzyme-linked immunosorbent assay for testing whether monoclonal antibodies recognize the same antigenic site. Application to hybridomas specific for the beta 2-subunit of Escherichia coli tryptophan synthase.

7. "Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A.

8. Distinction of epitopes by monoclonal antibodies.

9. Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide gels.

10. Characterisation and application of a murine monoclonal antibody specific for the serogroup C2 Salmonella.

1. Patterns of Reactivity between a Panel of Monoclonal Antibodies and Forage Rhizobium Strains.

2. Application of Recombinant Human scFv Antibody as a Powerful Tool to Monitor Nitrogen Fixing Biofertilizer in Rice and Legume.