Literature DB >> 1697149

Alternate gram staining technique using a fluorescent lectin.

R K Sizemore1, J J Caldwell, A S Kendrick.   

Abstract

Fluorescence-labeled wheat germ agglutinin binds specifically to N-acetylglucosamine in the outer peptidoglycan layer of gram-positive bacteria. The peptidoglycan layer of gram-negative bacteria is covered by a membrane and is not labeled by the lectin. By exploiting this phenomenon, an alternative Gram staining technique has been developed.

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Year:  1990        PMID： 1697149      PMCID： PMC184591          DOI： 10.1128/aem.56.7.2245-2247.1990

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  4 in total

1.  A New Modification and Application of the Gram Stain.

Authors:  G J Hucker
Journal:  J Bacteriol       Date:  1921-07       Impact factor: 3.490

2.  The Gram stain.

Authors:  J W BARTHOLOMEW; T MITTWER
Journal:  Bacteriol Rev       Date:  1952-03

3.  Wheat germ agglutinin. Molecular characteristics and specificity for sugar binding.

Authors:  Y Nagata; M M Burger
Journal:  J Biol Chem       Date:  1974-05-25       Impact factor: 5.157

4.  The purification, composition and specificity of wheat-germ agglutinin.

Authors:  A K Allen; A Neuberger; N Sharon
Journal:  Biochem J       Date:  1973-01       Impact factor: 3.857
  4 in total
  30 in total

1.  Evaluation of a fluorescent lectin-based staining technique for some acidophilic mining bacteria.

Authors:  D J Fife; D F Bruhn; K S Miller; D L Stoner
Journal:  Appl Environ Microbiol       Date:  2000-05       Impact factor: 4.792

2.  Rapid method for detection of gram-positive and -negative bacteria in milk from cows with moderate or severe clinical mastitis.

Authors:  S P Yazdankhah; H Sørum; H J Larsen; G Gogstad
Journal:  J Clin Microbiol       Date:  2001-09       Impact factor: 5.948

3.  Quantum dots as strain- and metabolism-specific microbiological labels.

Authors:  J A Kloepfer; R E Mielke; M S Wong; K H Nealson; G Stucky; J L Nadeau
Journal:  Appl Environ Microbiol       Date:  2003-07       Impact factor: 4.792

4.  Simultaneous fluorescent gram staining and activity assessment of activated sludge bacteria.

Authors:  Scott Forster; Jason R Snape; Hilary M Lappin-Scott; Jonathan Porter
Journal:  Appl Environ Microbiol       Date:  2002-10       Impact factor: 4.792

5.  Phylogenetic diversity, localization, and cell morphologies of members of the candidate phylum TG3 and a subphylum in the phylum Fibrobacteres, recently discovered bacterial groups dominant in termite guts.

Authors:  Yuichi Hongoh; Pinsurang Deevong; Satoshi Hattori; Tetsushi Inoue; Satoko Noda; Napavarn Noparatnaraporn; Toshiaki Kudo; Moriya Ohkuma
Journal:  Appl Environ Microbiol       Date:  2006-08-21       Impact factor: 4.792

6.  Cell wall peptidoglycan architecture in Bacillus subtilis.

Authors:  Emma J Hayhurst; Lekshmi Kailas; Jamie K Hobbs; Simon J Foster
Journal:  Proc Natl Acad Sci U S A       Date:  2008-09-10       Impact factor: 11.205

Review 7.  Bacterial Vivisection: How Fluorescence-Based Imaging Techniques Shed a Light on the Inner Workings of Bacteria.

Authors:  Alexander Cambré; Abram Aertsen
Journal:  Microbiol Mol Biol Rev       Date:  2020-10-28       Impact factor: 11.056

8.  A flow-cytometric gram-staining technique for milk-associated bacteria.

Authors:  Claus Holm; Lene Jespersen
Journal:  Appl Environ Microbiol       Date:  2003-05       Impact factor: 4.792

9.  Use of an intelligent control system To evaluate multiparametric effects on iron oxidation by thermophilic bacteria

Authors: 
Journal:  Appl Environ Microbiol       Date:  1998-11       Impact factor: 4.792

10.  Transfer of microorganisms, including Listeria monocytogenes, from various materials to beef.

Authors:  Graziella Midelet; Brigitte Carpentier
Journal:  Appl Environ Microbiol       Date:  2002-08       Impact factor: 4.792
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