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Literature DB >> 16967933

Improved photostable FRET-competent biarsenical-tetracysteine probes based on fluorinated fluoresceins.

Carla C Spagnuolo¹, Rudolf J Vermeij, Elizabeth A Jares-Erijman.

Abstract

We have developed fluoro-substituted versions of the biarsenical-tetracysteine label FlAsH, exhibiting significant improvements in important properties over the original fluorescein derivative. In complexes with tetracysteine targets, F2FlAsH exhibits 50 times improved photostability, lower pH sensitivity, higher absorbance and quantum yield than FlAsH, and F4FlAsH adds a new color to the palette of biarsenical dyes. The two probes also provide a new FRET pair with a larger Ro value (54 A) than any previously obtained with biarsenical dyes.

Entities: Chemical

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Year: 2006 PMID： 16967933 DOI： 10.1021/ja063212q

Source DB: PubMed Journal: J Am Chem Soc ISSN： 0002-7863 Impact factor: 15.419

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Cited

17 in total

10. Enhanced photostability of genetically encodable fluoromodules based on fluorogenic cyanine dyes and a promiscuous protein partner.

Authors: Nathaniel I Shank; Kimberly J Zanotti; Frederick Lanni; Peter B Berget; Bruce A Armitage
Journal: J Am Chem Soc Date: 2009-09-16 Impact factor: 15.419

Improved photostable FRET-competent biarsenical-tetracysteine probes based on fluorinated fluoresceins.

Review 1. Application of metal coordination chemistry to explore and manipulate cell biology.

2. Studying the dynamics of flagella in multicellular communities of Escherichia coli by using biarsenical dyes.

3. Gα13 Switch Region 2 Relieves Talin Autoinhibition to Activate αIIbβ3 Integrin.

4. Surveying protein structure and function using bis-arsenical small molecules.

5. Imaging nanometer-sized α-synuclein aggregates by superresolution fluorescence localization microscopy.

6. Fluorescent labeling of tetracysteine-tagged proteins in intact cells.

7. Rotational diffusion of the α(2a) adrenergic receptor revealed by FlAsH labeling in living cells.

8. Probing the target-specific inhibition of sensitized protein tyrosine phosphatases with biarsenical probes.

9. Biarsenical-tetracysteine motif as a fluorescent tag for detection in capillary electrophoresis.

10. Enhanced photostability of genetically encodable fluoromodules based on fluorogenic cyanine dyes and a promiscuous protein partner.