| Literature DB >> 1696550 |
A Schweiger1, O Mihalache, A Muhr, I Adrian.
Abstract
Phosphotyrosine-containing proteins in Dictyostelium discoideum were detected by immunoblot analysis and immunoprecipitation using a monoclonal anti-phosphotyrosine antibody. The iodinated antibody recognized on bots a cluster of 205-220 kDa polypeptides and bands of 107 and 60 kDa. The 107 and 60 kDa polypeptides and, in addition, a 82 kDa one became phosphorylated on tyrosine when the immunoprecipitate was incubated with [gamma-32P]ATP. In preparations from differentiating cells the intensity of the label was increased in the 60 kDa band and decreased in the 107 and 205-220 kDa bands.Entities:
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Year: 1990 PMID： 1696550 DOI： 10.1016/0014-5793(90)81007-b
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124