| Literature DB >> 16964386 |
Jonathan Tammela1, Akiko Uenaka, Toshiro Ono, Yuji Noguchi, Achim A Jungbluth, Paulette Mhawech-Fauceglia, Feng Qian, Sally Schneider, Sameer Sharma, Deborah Driscoll, Shashikant Lele, Lloyd J Old, Eiichi Nakayama, Kunle Odunsi.
Abstract
OY-TES-1 is a novel target that belongs to the family of 'cancer/testis' (CT) antigens. Our goal was to examine the expression and immunogenicity of OY-TES-1 in epithelial ovarian cancer (EOC) to determine its potential as a target for vaccine therapy. OY-TES-1 expression was determined by one-step reverse transcriptase PCR on 100 EOC samples, 5 EOC cell lines, and a panel of normal tissues. Immunohistochemistry (IHC) was performed on the same panel of EOC tissues. Sera from a sub-group of patients were tested for OY-TES-1 antibody by ELISA. Thymus and leukocytes were weakly positive for OY-TES-1 while the remaining 5 normal tissues were negative. Expression of OY-TES-1 by either RT-PCR and/or IHC was demonstrable in 69/100 (69%) tumors. Humoral immunity to OY-TES-1 was demonstrated in 1/10 (10%) serum samples from patients whose tumors expressed the antigen. The median follow-up of the patient population was 34 months. There was no correlation between antigen expression and stage, grade, histology and survival. OY-TES-1 is expressed in 69% of patients with EOC, is absent from normal ovarian tissue, and a proportion of patients show evidence of a specific humoral immune response. These findings make OY-TES-1 an attractive target for antigen-specific immunotherapy in EOC.Entities:
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Year: 2006 PMID： 16964386
Source DB: PubMed Journal: Int J Oncol ISSN： 1019-6439 Impact factor: 5.650