BACKGROUND: In Brazil, dengue endemic and epidemic patterns indicate an upward trend in incidence and hospitalization in the past decade. OBJECTIVE: To report dengue circulating serotypes from 1994 to 2003 and the role of distinct serotypes on dengue clinical outcomes in Central Brazil. METHODS: Virological surveillance for dengue cases was conducted in the city of Goiania ( approximately 1,200,000 population) from 1994 to 2003. Samples were tested using dengue IgM antibody (MAC-ELISA) and/or virus isolation. Circulating subtypes and genotypes were identified by reverse transcriptase PCR (RT-PCR) and by restricted site-specific PCR (RSS-PCR) patterns in selected samples. RESULTS: Adults (87.4%) were the most affected group and dengue fever accounted for the majority of the cases. Laboratory surveillance identified mainly DEN 1 serotype from 1994 to 2002 shifting to a high circulation of DEN 3 in 2003. The ratio of dengue fever to dengue with complications/DHF remained constant following the introduction of DEN 3. Diagnosis of dengue was confirmed in approximately 50% of the suspected cases enhanced by RT-PCR. RSS-PCR patterns for DEN 1 and DEN 3 corresponded to the circulating subtypes in the country. CONCLUSIONS: The result of virological surveillance did not suggest a major role of infecting DEN 3 serotype in increasing disease severity during its first-year spread in Central Brazil.
BACKGROUND: In Brazil, dengue endemic and epidemic patterns indicate an upward trend in incidence and hospitalization in the past decade. OBJECTIVE: To report dengue circulating serotypes from 1994 to 2003 and the role of distinct serotypes on dengue clinical outcomes in Central Brazil. METHODS: Virological surveillance for dengue cases was conducted in the city of Goiania ( approximately 1,200,000 population) from 1994 to 2003. Samples were tested using dengue IgM antibody (MAC-ELISA) and/or virus isolation. Circulating subtypes and genotypes were identified by reverse transcriptase PCR (RT-PCR) and by restricted site-specific PCR (RSS-PCR) patterns in selected samples. RESULTS: Adults (87.4%) were the most affected group and dengue fever accounted for the majority of the cases. Laboratory surveillance identified mainly DEN 1 serotype from 1994 to 2002 shifting to a high circulation of DEN 3 in 2003. The ratio of dengue fever to dengue with complications/DHF remained constant following the introduction of DEN 3. Diagnosis of dengue was confirmed in approximately 50% of the suspected cases enhanced by RT-PCR. RSS-PCR patterns for DEN 1 and DEN 3 corresponded to the circulating subtypes in the country. CONCLUSIONS: The result of virological surveillance did not suggest a major role of infecting DEN 3 serotype in increasing disease severity during its first-year spread in Central Brazil.
Authors: Osama M E Seidahmed; Safa A Hassan; Mohamed A Soghaier; Hanna A M Siam; Fayez T A Ahmed; Mubarak M Elkarsany; Suad M Sulaiman Journal: PLoS Negl Trop Dis Date: 2012-09-27
Authors: Angela F L T Argolo; Valéria C R Féres; Lucimeire A Silveira; Anna Carolina M Oliveira; Luiz A Pereira; João Bosco Siqueira Júnior; Cynthia Braga; Celina M T Martelli Journal: BMC Infect Dis Date: 2013-05-31 Impact factor: 3.090
Authors: João B Siqueira-Junior; Ivan J Maciel; Christovam Barcellos; Wayner V Souza; Marilia S Carvalho; Nazareth E Nascimento; Renato M Oliveira; Otaliba Morais-Neto; Celina M T Martelli Journal: BMC Public Health Date: 2008-10-20 Impact factor: 3.295
Authors: Benigno A M Rocha; Adriana O Guilarde; Angela F L T Argolo; Marianna Peres Tassara; Lucimeire A da Silveira; Isabela C Junqueira; Marília D Turchi; Valéria C R Féres; Celina M T Martelli Journal: Infect Dis Poverty Date: 2017-08-02 Impact factor: 4.520