| Literature DB >> 16960364 |
Yoshikazu Fujii1, Shinji Hirosue, Tadashi Fujii, Naoki Matsumoto, Hitosi Agematu, Akira Arisawa.
Abstract
A gene for cytochrome P450 (moxA) from Nonomuraea recticatena, coexpressed with camAB for pseudomonad redox partners in Escherichia coli, hydroxylated oleanolic acid to produce queretaroic acid. When we used the P450-induced whole-cell as a catalyst, only a small amount of queretaroic acid was produced, probably due to poor permeability of oleanolic acid into the E. coli cell. In an alternative approach with the cell-free reaction system, the conversion ratio increased up to 17%.Entities:
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Year: 2006 PMID： 16960364 DOI： 10.1271/bbb.60126
Source DB: PubMed Journal: Biosci Biotechnol Biochem ISSN： 0916-8451 Impact factor: 2.043