| Literature DB >> 16957366 |
Abstract
MicroRNAs (miRNAs) are approx 22-nucleotide (nt)-long, single-stranded, endogenous, noncoding RNAs that are widely expressed in multicellular organisms. This chapter describes methods that allow the overexpression of human miRNAs and also discusses how primary miRNAs (pri-miRNAs), the much longer precursors of mature miRNAs, are processed in human cells, as well as in vitro.Entities:
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Year: 2006 PMID： 16957366 DOI： 10.1385/1-59745-123-1:49
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745