| Literature DB >> 16957259 |
N R Mullane1, J Murray, D Drudy, N Prentice, P Whyte, P G Wall, A Parton, S Fanning.
Abstract
Enterobacter sakazakii has been associated with life-threatening infections in premature low-birth-weight infants. Contaminated infant milk formula (IMF) has been implicated in cases of E. sakazakii meningitis. Quick and sensitive methods to detect low-level contamination sporadically present in IMF preparations would positively contribute towards risk reduction across the infant formula food chain. Here we report on the development of a simple method, combining charged separation and growth on selective agar, to detect E. sakazakii in IMF. This protocol can reliably detect 1 to 5 CFU of E. sakazakii in 500 g of IMF in less than 24 h.Entities:
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Year: 2006 PMID： 16957259 PMCID： PMC1563671 DOI： 10.1128/AEM.03056-05
Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792