| Literature DB >> 16956687 |
Lars Forsberg1, Catharina Larsson, Anastasios Sofiadis, Rolf Lewensohn, Anders Höög, Janne Lehtiö.
Abstract
We report a protocol for pre-fractionation of proteins from frozen tumours. Using this protocol two separate protein fractions are extracted: Fraction 1 is enriched for cytosolic and Fraction 2 for nuclear/nuclear membrane proteins. The accuracy and reproducibility of the protocol were demonstrated by Western blot analyses and SELDI-TOF-MS profilings. The resulting protein profiles were similar within the respective groups of Fractions 1 and 2 samples, without differences between freshly prepared tumours or those obtained as frozen tissue from biobanks.Entities:
Mesh：
Year: 2006 PMID： 16956687 DOI： 10.1016/j.jbiotec.2006.05.020
Source DB: PubMed Journal: J Biotechnol ISSN： 0168-1656 Impact factor: 3.307