Literature DB >> 1695573

Comparison of four stains and a urease test for rapid detection of Helicobacter pylori in gastric biopsies.

A E Simor1, N B Cooter, D E Low.   

Abstract

Gastric biopsies were obtained from 125 subjects to compare detection of Helicobacter pylori by culture, a rapid urease test and histopathologic examination using haematoxylin-eosin, Gram, Giemsa, Warthin-Starry silver and acridine orange stains. Helicobacter pylori was isolated from 39 specimens. Acridine orange and Giemsa were the most sensitive stains, detecting 85% and 79% of positive specimens respectively. All stains showed high specificity (97-100%). The sensitivity and specificity of the rapid urease test was 62% and 100% respectively. These stains or the rapid urease test may be useful for rapid detection of Helicobacter pylori in gastric biopsies.

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Year:  1990        PMID： 1695573     DOI： 10.1007/bf01973743

Source DB:  PubMed          Journal:  Eur J Clin Microbiol Infect Dis        ISSN： 0934-9723            Impact factor:   3.267


  12 in total

1.  Campylobacter pyloridis-associated chronic active antral gastritis. A prospective study of its prevalence and the effects of antibacterial and antiulcer treatment.

Authors:  E A Rauws; W Langenberg; H J Houthoff; H C Zanen; G N Tytgat
Journal:  Gastroenterology       Date:  1988-01       Impact factor: 22.682

2.  Rapid diagnosis of Campylobacter pyloridis gastritis.

Authors:  C A McNulty; R Wise
Journal:  Lancet       Date:  1986-02-15       Impact factor: 79.321

3.  Comparison of rapid urease tests, staining techniques, and growth on different solid media for detection of Campylobacter pylori.

Authors:  P E Coudron; D F Kirby
Journal:  J Clin Microbiol       Date:  1989-07       Impact factor: 5.948

4.  Rapid urease test in the management of Campylobacter pyloridis-associated gastritis.

Authors:  B J Marshall; J R Warren; G J Francis; S R Langton; C S Goodwin; E D Blincow
Journal:  Am J Gastroenterol       Date:  1987-03       Impact factor: 10.864

5.  Campylobacter pylori antibodies in humans.

Authors:  G I Perez-Perez; B M Dworkin; J E Chodos; M J Blaser
Journal:  Ann Intern Med       Date:  1988-07-01       Impact factor: 25.391

6.  Association of Campylobacter pylori on the gastric mucosa with antral gastritis in children.

Authors:  B Drumm; P Sherman; E Cutz; M Karmali
Journal:  N Engl J Med       Date:  1987-06-18       Impact factor: 91.245

Review 7.  Gastric Campylobacter-like organisms, gastritis, and peptic ulcer disease.

Authors:  M J Blaser
Journal:  Gastroenterology       Date:  1987-08       Impact factor: 22.682

8.  Rapid diagnosis of Campylobacter pylori by Gram's stain.

Authors:  E A Montgomery; D F Martin; D A Peura
Journal:  Am J Clin Pathol       Date:  1988-11       Impact factor: 2.493

9.  Indirect immunofluorescence test and enzyme-linked immunosorbent assay for detection of Campylobacter pylori.

Authors:  E Schaber; F Umlauft; G Stöffler; F Aigner; B Paulweber; F Sandhofer
Journal:  J Clin Microbiol       Date:  1989-02       Impact factor: 5.948

10.  Simple microbiologic detection of Campylobacter pylori.

Authors:  J Parsonnet; K Welch; C Compton; R Strauss; T Wang; P Kelsey; M J Ferraro
Journal:  J Clin Microbiol       Date:  1988-05       Impact factor: 5.948
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  2 in total

1.  Detection of Helicobacter pylori in gastric biopsy tissue by polymerase chain reaction.

Authors:  J T Wang; J T Lin; J C Sheu; J C Yang; D S Chen; T H Wang
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1993-05       Impact factor: 3.267

2.  Sensitive detection of Helicobacter pylori by using polymerase chain reaction.

Authors:  C L Clayton; H Kleanthous; P J Coates; D D Morgan; S Tabaqchali
Journal:  J Clin Microbiol       Date:  1992-01       Impact factor: 5.948
  2 in total

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