Literature DB >> 16952840

Performance of expression vector, pTD1, in insect cell-free translation system.

Takashi Suzuki1, Masaaki Ito, Toru Ezure, Shinichiro Kobayashi, Masamitsu Shikata, Koji Tanimizu, Osamu Nishimura.   

Abstract

We constructed a pTD1 vector for an insect cell-free translation system containing a 5' untranslated region (UTR) of a polyhedrin gene as a translational enhancer sequence. Its translational efficiency was about 50-fold higher than those of mRNAs without an enhancer sequence. Moreover, the pTD1 vector functioned as an effective expression vector not only in the insect cell-free translation system but also in wheat germ extract and rabbit reticulocyte lysate systems.

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Year:  2006        PMID： 16952840     DOI： 10.1263/jbb.102.69

Source DB:  PubMed          Journal:  J Biosci Bioeng        ISSN： 1347-4421            Impact factor:   2.894


  11 in total

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8.  Identification of Human N-Myristoylated Proteins from Human Complementary DNA Resources by Cell-Free and Cellular Metabolic Labeling Analyses.

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10.  Tobacco BY-2 cell-free lysate: an alternative and highly-productive plant-based in vitro translation system.

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