Literature DB >> 1694967

Activity profiles of enzymes that control the uracil incorporation into DNA during neuronal development.

F Focher1, P Mazzarello, A Verri, U Hübscher, S Spadari.   

Abstract

We have shown that DNA polymerase beta, the only nuclear DNA polymerase present in adult neurons, cannot discriminate between dTTP and dUTP, having the same Km for both substrates. This fact suggests that during reparative DNA synthesis, in adult neurons, dUMP residues can be incorporated into DNA. Since uracil DNA-glycosylase functions to prevent the mutagenic effects of uracil in DNA coming as a product of deamination of cytosine residues or as a result of dUMP incorporation by DNA polymerase, we have studied the perinatal activity of uracil DNA-glycosylase and of 2 enzymes (nucleoside diphosphokinase and dUTPase) involved in dUTP metabolism. Our data indicate that during neuronal development there is a rapid decrease in uracil DNA-glycosylase which could impair the removal of uracil present in DNA in adult neurons. However, misincorporation of dUMP into DNA might be kept to a low frequency by the action of dUTPase present at all developmental stages.

Entities:  

Mesh：

Substances：

Year:  1990        PMID： 1694967     DOI： 10.1016/0921-8734(90)90012-g

Source DB:  PubMed          Journal:  Mutat Res        ISSN： 0027-5107            Impact factor:   2.433


  23 in total

1.  The replicative DNA polymerase of herpes simplex virus 1 exhibits apurinic/apyrimidinic and 5'-deoxyribose phosphate lyase activities.

Authors:  Federica Bogani; Paul E Boehmer
Journal:  Proc Natl Acad Sci U S A       Date:  2008-08-11       Impact factor: 11.205

2.  The specific binding of nuclear protein(s) to the cAMP responsive element (CRE) sequence (TGACGTCA) is reduced by the misincorporation of U and increased by the deamination of C.

Authors:  A Verri; P Mazzarello; G Biamonti; S Spadari; F Focher
Journal:  Nucleic Acids Res       Date:  1990-10-11       Impact factor: 16.971

3.  Mutations in active-site residues of the uracil-DNA glycosylase encoded by vaccinia virus are incompatible with virus viability.

Authors:  K S Ellison; W Peng; G McFadden
Journal:  J Virol       Date:  1996-11       Impact factor: 5.103

4.  Absence of the uracil DNA glycosylase of murine gammaherpesvirus 68 impairs replication and delays the establishment of latency in vivo.

Authors:  Nana Minkah; Marc Macaluso; Darby G Oldenburg; Clinton R Paden; Douglas W White; Kevin M McBride; Laurie T Krug
Journal:  J Virol       Date:  2015-01-14       Impact factor: 5.103

5.  Evidence that the herpes simplex virus type 1 uracil DNA glycosylase is required for efficient viral replication and latency in the murine nervous system.

Authors:  R B Pyles; R L Thompson
Journal:  J Virol       Date:  1994-08       Impact factor: 5.103

6.  Stereospecificity of human DNA polymerases alpha, beta, gamma, delta and epsilon, HIV-reverse transcriptase, HSV-1 DNA polymerase, calf thymus terminal transferase and Escherichia coli DNA polymerase I in recognizing D- and L-thymidine 5'-triphosphate as substrate.

Authors:  F Focher; G Maga; A Bendiscioli; M Capobianco; F Colonna; A Garbesi; S Spadari
Journal:  Nucleic Acids Res       Date:  1995-08-11       Impact factor: 16.971

7.  Replication in vitro and in vivo of an equine infectious anemia virus mutant deficient in dUTPase activity.

Authors:  D L Lichtenstein; K E Rushlow; R F Cook; M L Raabe; C J Swardson; G J Kociba; C J Issel; R C Montelaro
Journal:  J Virol       Date:  1995-05       Impact factor: 5.103

8.  Expression of mitochondrial genes and DNA-repair-related nuclear genes is altered in xeroderma pigmentosum fibroblasts.

Authors:  X Xia; D Werner; O Popanda; H W Thielmann
Journal:  J Cancer Res Clin Oncol       Date:  1994       Impact factor: 4.553

9.  Uracil-DNA glycosylases preferentially excise mispaired uracil.

Authors:  A Verri; P Mazzarello; S Spadari; F Focher
Journal:  Biochem J       Date:  1992-11-01       Impact factor: 3.857

10.  Uracil in OriS of herpes simplex 1 alters its specific recognition by origin binding protein (OBP): does virus induced uracil-DNA glycosylase play a key role in viral reactivation and replication?

Authors:  F Focher; A Verri; S Verzeletti; P Mazzarello; S Spadari
Journal:  Chromosoma       Date:  1992       Impact factor: 4.316
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.