Literature DB >> 1694789

The role of a conserved guanosine residue in the hammerhead-type RNA enzyme.

O Odai1, H Hiroaki, T Sakata, T Tanaka, S Uesugi.   

Abstract

We have designed a hammerhead-type RNA system which consists of three RNA fragments for normal and modified complexes which contain a non-cleavable substrate with 2'-O-methylcytidine and a guanosine-to-inosine replaced enzyme. Examination of the RNA-cleaving activity and conformational properties of the complexes suggests that the 2-amino group of a conserved guanosine residue in the loop region plays an important role for maintaining both the activity and loop conformation.

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Year:  1990        PMID： 1694789     DOI： 10.1016/0014-5793(90)80311-6

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  8 in total

1.  Enhancement of ribozyme catalytic activity by a contiguous oligodeoxynucleotide (facilitator) and by 2'-O-methylation.

Authors:  J Goodchild
Journal:  Nucleic Acids Res       Date:  1992-09-11       Impact factor: 16.971

2.  Reconstitution of enzymatic activity from fragments of M1 RNA.

Authors:  C Guerrier-Takada; S Altman
Journal:  Proc Natl Acad Sci U S A       Date:  1992-02-15       Impact factor: 11.205

3.  Newt satellite 2 transcripts self-cleave by using an extended hammerhead structure.

Authors:  L M Pabón-Peña; Y Zhang; L M Epstein
Journal:  Mol Cell Biol       Date:  1991-12       Impact factor: 4.272

4.  Importance of specific purine amino and hydroxyl groups for efficient cleavage by a hammerhead ribozyme.

Authors:  D J Fu; L W McLaughlin
Journal:  Proc Natl Acad Sci U S A       Date:  1992-05-01       Impact factor: 11.205

5.  Total chemical synthesis of a ribozyme derived from a group I intron.

Authors:  S K Whoriskey; N Usman; J W Szostak
Journal:  Proc Natl Acad Sci U S A       Date:  1995-03-28       Impact factor: 11.205

6.  The synthesis of oligoribonucleotides containing O6-methylguanosine: the role of conserved guanosine residues in hammerhead ribozyme cleavage.

Authors:  J A Grasby; P Jonathan; G Butler; M J Gait
Journal:  Nucleic Acids Res       Date:  1993-09-25       Impact factor: 16.971

7.  Synthesis and NMR study of ribo-oligonucleotides forming a hammerhead-type RNA enzyme system.

Authors:  O Odai; H Kodama; H Hiroaki; T Sakata; T Tanaka; S Uesugi
Journal:  Nucleic Acids Res       Date:  1990-10-25       Impact factor: 16.971

8.  Synthesis, deprotection, analysis and purification of RNA and ribozymes.

Authors:  F Wincott; A DiRenzo; C Shaffer; S Grimm; D Tracz; C Workman; D Sweedler; C Gonzalez; S Scaringe; N Usman
Journal:  Nucleic Acids Res       Date:  1995-07-25       Impact factor: 16.971
  8 in total

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