Literature DB >> 16946480

Crystallization of mutant forms of glutaredoxin Grx1p from yeast.

Kjell O Håkansson1, Henrik Østergaard, Jakob R Winther.   

Abstract

Glutaredoxin Grx1p from yeast was crystallized both as an independent protein and in a protein fusion with His-tagged yellow fluorescent protein (rxYFP). A glutathionylated C30S mutant of the 12 kDa Grx1p was crystallized in two different forms in PEG 4000 at low pH. These orthorhombic and monoclinic forms diffract to 2.0 A (synchrotron radiation) and 2.7 A (rotating-anode generator), respectively. In contrast, rxYFP-Grx1p formed crystals at high pH in MgSO(4) which diffract synchrotron radiation to 2.7 A.

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Year:  2006        PMID： 16946480      PMCID： PMC2242864          DOI： 10.1107/S1744309106031216

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  9 in total

1.  The Protein Data Bank.

Authors:  H M Berman; J Westbrook; Z Feng; G Gilliland; T N Bhat; H Weissig; I N Shindyalov; P E Bourne
Journal:  Nucleic Acids Res       Date:  2000-01-01       Impact factor: 16.971

2.  The CCP4 suite: programs for protein crystallography.

Authors: 
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  1994-09-01

3.  Solvent content of protein crystals.

Authors:  B W Matthews
Journal:  J Mol Biol       Date:  1968-04-28       Impact factor: 5.469

4.  Shedding light on disulfide bond formation: engineering a redox switch in green fluorescent protein.

Authors:  H Ostergaard; A Henriksen; F G Hansen; J R Winther
Journal:  EMBO J       Date:  2001-11-01       Impact factor: 11.598

5.  Structure of oxidized bacteriophage T4 glutaredoxin (thioredoxin). Refinement of native and mutant proteins.

Authors:  H Eklund; M Ingelman; B O Söderberg; T Uhlin; P Nordlund; M Nikkola; U Sonnerstam; T Joelson; K Petratos
Journal:  J Mol Biol       Date:  1992-11-20       Impact factor: 5.469

6.  Sequence-specific 1H n.m.r. assignments and determination of the three-dimensional structure of reduced Escherichia coli glutaredoxin.

Authors:  P Sodano; T H Xia; J H Bushweller; O Björnberg; A Holmgren; M Billeter; K Wüthrich
Journal:  J Mol Biol       Date:  1991-10-20       Impact factor: 5.469

Review 7.  Glutaredoxins: glutathione-dependent redox enzymes with functions far beyond a simple thioredoxin backup system.

Authors:  Aristi Potamitou Fernandes; Arne Holmgren
Journal:  Antioxid Redox Signal       Date:  2004-02       Impact factor: 8.401

8.  Mechanistic insight provided by glutaredoxin within a fusion to redox-sensitive yellow fluorescent protein.

Authors:  Olof Björnberg; Henrik Østergaard; Jakob R Winther
Journal:  Biochemistry       Date:  2006-02-21       Impact factor: 3.162

9.  The tetrameric structure of Haemophilus influenza hybrid Prx5 reveals interactions between electron donor and acceptor proteins.

Authors:  Seung Jun Kim; Joo Rang Woo; Young Sun Hwang; Dae Gwin Jeong; Dong Hae Shin; Kanghwa Kim; Seong Eon Ryu
Journal:  J Biol Chem       Date:  2003-01-14       Impact factor: 5.157
  9 in total

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