| Literature DB >> 16945400 |
Sukumar Saha1, Shinsuke Yoshida, Kenji Ohba, Kiyohiko Matsui, Tomoko Matsuda, Fumihiko Takeshita, Kazunori Umeda, Yuichi Tamura, Kentaro Okuda, Dennis Klinman, Ke-Qin Xin, Kenji Okuda.
Abstract
We evaluated the immunogenicity and protective activity of plasmid DNA vaccines encoding the influenza virus NP gene (pNP) alone or in combination with the herpes simplex virus type 1 protein 22 gene (pVP22). Optimal immune responses were observed in BALB/c mice immunized with the combination of pVP22 plus pNP, as assessed by enzyme-linked immunosorbent assay (ELISA), enzyme-linked immunospot (ELISPOT) and intracellular cytokine staining (ICCS). These mice also showed maximal resistance following challenge with the A/PR/8/34 (H1N1) and A/Udron/72 (H3N2) strains of influenza virus. The susceptibility of immunized mice to virus infection was significantly increased following depletion of either CD4+ or CD8+ T cells. These results indicate that a plasmid DNA vaccine encoding pVP22 plus NP induces a high level of cross-protective immunity against influenza virus subtypes.Entities:
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Year: 2006 PMID： 16945400 DOI： 10.1016/j.virol.2006.04.015
Source DB: PubMed Journal: Virology ISSN： 0042-6822 Impact factor: 3.616