Literature DB >> 16941243

Discovery of Bacillus thuringiensis virulence genes using signature-tagged mutagenesis in an insect model of septicaemia.

James R Steggles1, Jinhong Wang, David J Ellar.   

Abstract

Transposon Tn917 was used to identify Bacillus thuringiensis genes required for virulence and survival in a Manduca sexta (tobacco hornworm) septicaemia model. Uniquely tagged transposons, n = 72, were constructed and used to generate 1152 insertion mutants. Sixteen pools of 72 mutants were screened in the infection model, and 12 virulence-attenuated mutants were unable to survive the infection. Analysis of the mutated DNA sequences implicated an arsR family transcriptional regulator, a histone-like DNA-binding protein, a transposon, and several sequences of unknown function in B. thuringiensis pathogenesis.

Entities:  

Mesh：

Substances：

Year:  2006        PMID： 16941243     DOI： 10.1007/s00284-006-0037-2

Source DB:  PubMed          Journal:  Curr Microbiol        ISSN： 0343-8651            Impact factor:   2.188


  29 in total

1.  Multiple roles for TnpI recombinase in regulation of Tn5401 transposition in Bacillus thuringiensis.

Authors:  J A Baum; A J Gilmer; A M Light Mettus
Journal:  J Bacteriol       Date:  1999-10       Impact factor: 3.490

Review 2.  Lessons from signature-tagged mutagenesis on the infectious mechanisms of pathogenic bacteria.

Authors:  Nicolas Autret; Alain Charbit
Journal:  FEMS Microbiol Rev       Date:  2004-11-26       Impact factor: 16.408

3.  Identification of Yersinia enterocolitica genes affecting survival in an animal host using signature-tagged transposon mutagenesis.

Authors:  A J Darwin; V L Miller
Journal:  Mol Microbiol       Date:  1999-04       Impact factor: 3.501

4.  DNA replication in Escherichia coli mutants that lack protein HU.

Authors:  T Ogawa; M Wada; Y Kano; F Imamoto; T Okazaki
Journal:  J Bacteriol       Date:  1989-10       Impact factor: 3.490

5.  A plasmid-encoded regulator couples the synthesis of toxins and surface structures in Bacillus anthracis.

Authors:  Tâm Mignot; Michèle Mock; Agnès Fouet
Journal:  Mol Microbiol       Date:  2003-02       Impact factor: 3.501

Review 6.  Bacillus thuringiensis and its pesticidal crystal proteins.

Authors:  E Schnepf; N Crickmore; J Van Rie; D Lereclus; J Baum; J Feitelson; D R Zeigler; D H Dean
Journal:  Microbiol Mol Biol Rev       Date:  1998-09       Impact factor: 11.056

7.  Staphylococcus aureus genetic loci impacting growth and survival in multiple infection environments.

Authors:  S N Coulter; W R Schwan; E Y Ng; M H Langhorne; H D Ritchie; S Westbrock-Wadman; W O Hufnagle; K R Folger; A S Bayer; C K Stover
Journal:  Mol Microbiol       Date:  1998-10       Impact factor: 3.501

Review 8.  Bacillus cereus and related species.

Authors:  F A Drobniewski
Journal:  Clin Microbiol Rev       Date:  1993-10       Impact factor: 26.132

9.  Overproduction of encapsulated insecticidal crystal proteins in a Bacillus thuringiensis spo0A mutant.

Authors:  D Lereclus; H Agaisse; M Gominet; J Chaufaux
Journal:  Biotechnology (N Y)       Date:  1995-01

10.  Structural and functional analysis of Tn4430: identification of an integrase-like protein involved in the co-integrate-resolution process.

Authors:  J Mahillon; D Lereclus
Journal:  EMBO J       Date:  1988-05       Impact factor: 11.598
View more
  3 in total

1.  Effects of the Bacillus thuringiensis toxin Cry1Ab on membrane currents of isolated cells of the ruminal epithelium.

Authors:  Friederike Stumpff; Angelika Bondzio; Ralf Einspanier; Holger Martens
Journal:  J Membr Biol       Date:  2007-08-05       Impact factor: 1.843

2.  Whole-genome phylogenies of the family Bacillaceae and expansion of the sigma factor gene family in the Bacillus cereus species-group.

Authors:  Timothy R Schmidt; Edgar J Scott; David W Dyer
Journal:  BMC Genomics       Date:  2011-08-24       Impact factor: 3.969

3.  Divergence of the SigB regulon and pathogenesis of the Bacillus cereus sensu lato group.

Authors:  Edgar Scott; David W Dyer
Journal:  BMC Genomics       Date:  2012-10-22       Impact factor: 3.969
  3 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.