Literature DB >> 16941059

SYBR Green real-time reverse transcription-polymerase chain reaction assay for the generic detection of coronaviruses.

S Escutenaire1, N Mohamed, M Isaksson, P Thorén, B Klingeborn, S Belák, M Berg, J Blomberg.   

Abstract

Coronaviruses are etiologic agents of respiratory and enteric diseases in humans and in animals. In this study, a one-step real-time reverse transcription-polymerase chain reaction (RT-PCR) assay based on SYBR Green chemistry and degenerate primers was developed for the generic detection of coronaviruses. The primers, designed in the open reading frame 1b, enabled the detection of 32 animal coronaviruses including strains of canine coronavirus, feline coronavirus, transmissible gastroenteritis virus (TGEV), bovine coronavirus (BCoV), murine hepatitis virus (MHV) and infectious bronchitis virus (IBV). A specific amplification was also observed with the human coronaviruses (HCoV) HCoV-NL63, HCoV-OC43, HCoV-229E and severe acute respiratory syndrome coronavirus (SARS-CoV). The real-time RT-PCR detected down to 10 cRNA copies from TGEV, BCoV, SARS-CoV and IBV. In addition, the assay exhibited a high sensitivity and specificity on clinical samples from different animal species. The developed assay represents a potential tool for laboratory diagnostics and for detecting still uncharacterized coronaviruses.

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Year:  2006        PMID： 16941059      PMCID： PMC7087200          DOI： 10.1007/s00705-006-0840-x

Source DB:  PubMed          Journal:  Arch Virol        ISSN： 0304-8608            Impact factor:   2.574


  36 in total

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Journal:  J Clin Microbiol       Date:  2004-05       Impact factor: 5.948

2.  Genogroup I and II noroviruses detected in stool samples by real-time reverse transcription-PCR using highly degenerate universal primers.

Authors:  Gary P Richards; Michael A Watson; Rebecca L Fankhauser; Stephan S Monroe
Journal:  Appl Environ Microbiol       Date:  2004-12       Impact factor: 4.792

3.  Product differentiation by analysis of DNA melting curves during the polymerase chain reaction.

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Journal:  Anal Biochem       Date:  1997-02-15       Impact factor: 3.365

4.  Development and evaluation of a multitarget real-time Taqman reverse transcription-PCR assay for detection of the severe acute respiratory syndrome-associated coronavirus and surveillance for an apparently related coronavirus found in masked palm civets.

Authors:  Wenqian Hu; Bingke Bai; Zhihong Hu; Ze Chen; Xuefang An; Lijun Tang; Jihong Yang; Hualin Wang; Hanzhong Wang
Journal:  J Clin Microbiol       Date:  2005-05       Impact factor: 5.948

5.  A previously undescribed coronavirus associated with respiratory disease in humans.

Authors:  Ron A M Fouchier; Nico G Hartwig; Theo M Bestebroer; Berend Niemeyer; Jan C de Jong; James H Simon; Albert D M E Osterhaus
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6.  Mechanisms and enzymes involved in SARS coronavirus genome expression.

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Journal:  J Gen Virol       Date:  2003-09       Impact factor: 3.891

7.  An outbreak of coronavirus OC43 respiratory infection in Normandy, France.

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8.  Viral load quantitation of SARS-coronavirus RNA using a one-step real-time RT-PCR.

Authors:  Els Keyaerts; Leen Vijgen; Piet Maes; Griet Duson; Johan Neyts; Marc Van Ranst
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Authors:  C B Stephensen; D B Casebolt; N N Gangopadhyay
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10.  Development of a novel real-time RT-PCR assay with LUX primer for the detection of swine transmissible gastroenteritis virus.

Authors:  Ru Chen; Weiming Huang; Zhixiong Lin; Zhongfang Zhou; Haiqiong Yu; Daozhong Zhu
Journal:  J Virol Methods       Date:  2004-12-01       Impact factor: 2.014
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  38 in total

1.  Achieving a golden mean: mechanisms by which coronaviruses ensure synthesis of the correct stoichiometric ratios of viral proteins.

Authors:  Ewan P Plant; Rasa Rakauskaite; Deborah R Taylor; Jonathan D Dinman
Journal:  J Virol       Date:  2010-02-17       Impact factor: 5.103

2.  Diagnostic methods for feline coronavirus: a review.

Authors:  Saeed Sharif; Siti Suri Arshad; Mohd Hair-Bejo; Abdul Rahman Omar; Nazariah Allaudin Zeenathul; Amer Alazawy
Journal:  Vet Med Int       Date:  2010-07-28

3.  Prevalence and phylogeny of coronaviruses in wild birds from the Bering Strait area (Beringia).

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Journal:  PLoS One       Date:  2010-10-29       Impact factor: 3.240

4.  A real-time PCR assay for bat SARS-like coronavirus detection and its application to Italian greater horseshoe bat faecal sample surveys.

Authors:  Andrea Balboni; Laura Gallina; Alessandra Palladini; Santino Prosperi; Mara Battilani
Journal:  ScientificWorldJournal       Date:  2011-11-22

5.  Comparison of SYBR green I real-time RT-PCR with conventional agarose gel-based RT-PCR for the diagnosis of infectious bronchitis virus infection in chickens in Morocco.

Authors:  Siham Fellahi; Mehdi El Harrak; Jens H Kuhn; Ghizlane Sebbar; El Arbi Bouaiti; Khadija Khataby; Ouafae Fassi Fihri; Mohammed El Houadfi; My Mustapha Ennaji
Journal:  BMC Res Notes       Date:  2016-04-22

6.  Stability of bovine coronavirus on lettuce surfaces under household refrigeration conditions.

Authors:  Lisa Mullis; Linda J Saif; Yongbin Zhang; Xuming Zhang; Marli S P Azevedo
Journal:  Food Microbiol       Date:  2011-12-13       Impact factor: 5.516

7.  Human Respiratory Coronaviruses Detected In Patients with Influenza-Like Illness in Arkansas, USA.

Authors:  Camila S Silva; Lisa B Mullis; Olavo Pereira; Linda J Saif; Anastasia Vlasova; Xuming Zhang; Randall J Owens; Dale Paulson; Deborah Taylor; Lia M Haynes; Marli P Azevedo
Journal:  Virol Mycol       Date:  2014-03-26

8.  First molecular characterization of canine parvovirus strains in Sardinia, Italy.

Authors:  S Dei Giudici; T Cubeddu; A Giagu; G Sanna; S Rocca; A Oggiano
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9.  Low-cost HIV-1 diagnosis and quantification in dried blood spots by real time PCR.

Authors:  Nishaki Mehta; Sonia Trzmielina; Bareng A S Nonyane; Melissa N Eliot; Rongheng Lin; Andrea S Foulkes; Kristina McNeal; Arthur Ammann; Vindu Eulalievyolo; John L Sullivan; Katherine Luzuriaga; Mohan Somasundaran
Journal:  PLoS One       Date:  2009-06-05       Impact factor: 3.240

10.  Genomic Dissection of an Icelandic Epidemic of Respiratory Disease in Horses and Associated Zoonotic Cases.

Authors:  Sigríður Björnsdóttir; Simon R Harris; Vilhjálmur Svansson; Eggert Gunnarsson; Ólöf G Sigurðardóttir; Kristina Gammeljord; Karen F Steward; J Richard Newton; Carl Robinson; Amelia R L Charbonneau; Julian Parkhill; Matthew T G Holden; Andrew S Waller
Journal:  mBio       Date:  2017-08-01       Impact factor: 7.867
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