Literature DB >> 16937250

Enhanced production of recombinant nattokinase in Bacillus subtilis by the elimination of limiting factors.

Po Ting Chen1, Yun-Peng Chao.   

Abstract

By systematic investigation, glutamate and a mixture of metal ions were identified as factors limiting the production of nattokinase in Bacillus subtilis. Consequently, in medium supplemented with these materials, the recombinant strain secreted 4 times more nattokinase (260 mg l(-1)) than when grown in the unsupplemented medium.

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Year:  2006        PMID： 16937250     DOI： 10.1007/s10529-006-9126-3

Source DB:  PubMed          Journal:  Biotechnol Lett        ISSN： 0141-5492            Impact factor:   2.461


  4 in total

Review 1.  Microbial production of nattokinase: current progress, challenge and prospect.

Authors:  Dongbo Cai; Chengjun Zhu; Shouwen Chen
Journal:  World J Microbiol Biotechnol       Date:  2017-04-04       Impact factor: 3.312

2.  Cloning and enhancing production of a detergent- and organic-solvent-resistant nattokinase from Bacillus subtilis VTCC-DVN-12-01 by using an eight-protease-gene-deficient Bacillus subtilis WB800.

Authors:  Thao Thi Nguyen; Thi Dinh Quyen; Hoang Thanh Le
Journal:  Microb Cell Fact       Date:  2013-09-10       Impact factor: 5.328

3.  Study on the mechanism of production of γ-PGA and nattokinase in Bacillus subtilis natto based on RNA-seq analysis.

Authors:  Min Li; Zilong Zhang; Shenwei Li; Zhengan Tian; Xia Ma
Journal:  Microb Cell Fact       Date:  2021-04-09       Impact factor: 5.328

4.  Heterologous expression of nattokinase from B. subtilis natto using Pichia pastoris GS115 and assessment of its thrombolytic activity.

Authors:  Zhai Chao; Wang Yaping; Yan Guangbo; Shu Min; Shen Wei; Ma Lixin; Huang Zunxi
Journal:  BMC Biotechnol       Date:  2021-08-09       Impact factor: 2.563
  4 in total

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