Literature DB >> 16935253

Evidence of frequent integration of non-T-DNA vector backbone sequences in transgenic strawberry plant.

Samia Abdallah Abdal-Aziz1, Fernando Pliego-Alfaro, Miguel A Quesada, José A Mercado.   

Abstract

We have studied the occurrence of the integration of non-T-DNA sequences in transgenic strawberry plants obtained through Agrobacterium inoculation. DNA from these plants was subjected to PCR amplification of the sequence of the gene trfA, which is located outside the T-DNA. The percentage of trfA-positive plants varied from 40% to 90%, with a mean of 65.7%. Backbone sequences were confirmed by Southern blot analysis.

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Year:  2006        PMID： 16935253     DOI： 10.1263/jbb.101.508

Source DB:  PubMed          Journal:  J Biosci Bioeng        ISSN： 1347-4421            Impact factor:   2.894


  7 in total

1.  Comparison of DNA walking methods for isolation of transgene-flanking regions in GM potato.

Authors:  Danny Cullen; Wendy Harwood; Mark Smedley; Howard Davies; Mark Taylor
Journal:  Mol Biotechnol       Date:  2011-09       Impact factor: 2.695

2.  Vector integration in triple R gene transformants and the clustered inheritance of resistance against potato late blight.

Authors:  Suxian Zhu; Anita Duwal; Qi Su; Jack H Vossen; Richard G F Visser; Evert Jacobsen
Journal:  Transgenic Res       Date:  2012-08-31       Impact factor: 2.788

3.  Transformation of apple (Malus × domestica) using mutants of apple acetolactate synthase as a selectable marker and analysis of the T-DNA integration sites.

Authors:  Jia-Long Yao; Sumathi Tomes; Andrew P Gleave
Journal:  Plant Cell Rep       Date:  2013-03-15       Impact factor: 4.570

4.  Coexpression of octopine and succinamopine Agrobacterium virulence genes to generate high quality transgenic events in maize by reducing vector backbone integration.

Authors:  Nagesh Sardesai; Stephen Foulk; Wei Chen; Huixia Wu; Emily Etchison; Manju Gupta
Journal:  Transgenic Res       Date:  2018-10-06       Impact factor: 2.788

5.  Development of an efficient transformation method by Agrobacterium tumefaciens and high throughput spray assay to identify transgenic plants for woodland strawberry (Fragaria vesca) using NPTII selection.

Authors:  Christopher J Pantazis; Sarah Fisk; Kerri Mills; Barry S Flinn; Vladimir Shulaev; Richard E Veilleux; Yinghui Dan
Journal:  Plant Cell Rep       Date:  2012-11-16       Impact factor: 4.570

6.  Agrobacterium-mediated vacuum infiltration and floral dip transformation of rapid-cycling Brassica rapa.

Authors:  Die Hu; Andrew F Bent; Xilin Hou; Ying Li
Journal:  BMC Plant Biol       Date:  2019-06-10       Impact factor: 4.215

7.  An Insight into T-DNA Integration Events in Medicago sativa.

Authors:  Alessandro Nicolia; Nicoletta Ferradini; Fabio Veronesi; Daniele Rosellini
Journal:  Int J Mol Sci       Date:  2017-09-12       Impact factor: 5.923
  7 in total

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