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Literature DB >> 16935219

Gene silencing of serine proteases affects melanization of Sephadex beads in Anopheles gambiae.

Susan M Paskewitz¹, Olga Andreev, Lei Shi.

Abstract

Serine proteases play an important role in activation of prophenoloxidase (proPO), a critical enzyme in the production of melanin. We tested the effect of knockdown of gene expression for five clip domain serine proteases on melanization of abiotic targets in Anopheles gambiae. Knockdown of CLIPB4 resulted in a striking lack of melanization of Sephadex beads while knockdown of CLIPB8 caused a strong shift towards incompletely melanized beads. Knockdown of CLIPB1, B9 and B10 had lesser effects. CLIPB4 and CLIPB8 are strong candidates for activating enzymes in the proPO enzymatic cascade.

Entities: Chemical Species

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Year: 2006 PMID： 16935219 DOI： 10.1016/j.ibmb.2006.06.001

Source DB: PubMed Journal: Insect Biochem Mol Biol ISSN： 0965-1748 Impact factor: 4.714

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Cited

30 in total

1. Manduca sexta hemolymph proteinase 21 activates prophenoloxidase-activating proteinase 3 in an insect innate immune response proteinase cascade.

Authors: Maureen J Gorman; Yang Wang; Haobo Jiang; Michael R Kanost
Journal: J Biol Chem Date: 2007-02-22 Impact factor: 5.157

2. Cloning and molecular characterization of two invertebrate-type lysozymes from Anopheles gambiae.

Authors: S M Paskewitz; B Li; M K Kajla
Journal: Insect Mol Biol Date: 2008-04-07 Impact factor: 3.585

3. The viral protein Egf1.0 is a dual activity inhibitor of prophenoloxidase-activating proteinases 1 and 3 from Manduca sexta.

Authors: Zhiqiang Lu; Markus H Beck; Yang Wang; Haobo Jiang; Michael R Strand
Journal: J Biol Chem Date: 2008-06-02 Impact factor: 5.157

10. Genome-wide transcriptomic profiling of Anopheles gambiae hemocytes reveals pathogen-specific signatures upon bacterial challenge and Plasmodium berghei infection.

Authors: Luke A Baton; Anne Robertson; Emma Warr; Michael R Strand; George Dimopoulos
Journal: BMC Genomics Date: 2009-06-05 Impact factor: 3.969

Gene silencing of serine proteases affects melanization of Sephadex beads in Anopheles gambiae.

1. Manduca sexta hemolymph proteinase 21 activates prophenoloxidase-activating proteinase 3 in an insect innate immune response proteinase cascade.

2. Cloning and molecular characterization of two invertebrate-type lysozymes from Anopheles gambiae.

3. The viral protein Egf1.0 is a dual activity inhibitor of prophenoloxidase-activating proteinases 1 and 3 from Manduca sexta.

4. CLIPB8 is part of the prophenoloxidase activation system in Anopheles gambiae mosquitoes.

5. Serine protease-related proteins in the malaria mosquito, Anopheles gambiae.

6. Genome-wide profiling of diel and circadian gene expression in the malaria vector Anopheles gambiae.

7. Differentially expressed genes between female and male adult Anopheles anthropophagus.

8. Expression of Manduca sexta serine proteinase homolog precursors in insect cells and their proteolytic activation.

9. A novel polydnavirus protein inhibits the insect prophenoloxidase activation pathway.

10. Genome-wide transcriptomic profiling of Anopheles gambiae hemocytes reveals pathogen-specific signatures upon bacterial challenge and Plasmodium berghei infection.