Literature DB >> 1693515

Optimal conditions for synthesizing complementary DNA in the HIV-1 endogenous reverse transcriptase reaction.

W H Yong1, S Wyman, J A Levy.   

Abstract

Concentration of monovalent and divalent cations, anionic detergent, reducing agent and nucleotides, as well as pH, temperature, and incubation time were optimized for high levels of HIV-1 endogenous reverse transcriptase activity. In addition, mellitin, a peptide substitute for anionic detergent, and oligo(dT)12-18 were found to stimulate nucleic acid synthesis. This HIV-1 endogenous reaction demonstrated RNA- and DNA-dependent DNA polymerase activities. Nucleic acid intermediates and final products included RNA:DNA hybrids as well as single- and double-stranded DNA. The complementary DNA products formed were representative of all regions of the HIV-1 genome.

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Year:  1990        PMID： 1693515     DOI： 10.1097/00002030-199003000-00004

Source DB:  PubMed          Journal:  AIDS        ISSN： 0269-9370            Impact factor:   4.177


  23 in total

1.  Kinetics of human immunodeficiency virus type 1 reverse transcription in blood mononuclear phagocytes are slowed by limitations of nucleotide precursors.

Authors:  W A O'Brien; A Namazi; H Kalhor; S H Mao; J A Zack; I S Chen
Journal:  J Virol       Date:  1994-02       Impact factor: 5.103

2.  The site of antiviral action of 3-nitrosobenzamide on the infectivity process of human immunodeficiency virus in human lymphocytes.

Authors:  W G Rice; C A Schaeffer; L Graham; M Bu; J S McDougal; S L Orloff; F Villinger; M Young; S Oroszlan; M R Fesen
Journal:  Proc Natl Acad Sci U S A       Date:  1993-10-15       Impact factor: 11.205

3.  Amphipathic domains in the C terminus of the transmembrane protein (gp41) permeabilize HIV-1 virions: a molecular mechanism underlying natural endogenous reverse transcription.

Authors:  H Zhang; G Dornadula; P Alur; M A Laughlin; R J Pomerantz
Journal:  Proc Natl Acad Sci U S A       Date:  1996-10-29       Impact factor: 11.205

4.  Reverse transcriptase activity in chicken embryo fibroblast culture supernatants is associated with particles containing endogenous avian retrovirus EAV-0 RNA.

Authors:  R N Weissmahr; J Schüpbach; J Böni
Journal:  J Virol       Date:  1997-04       Impact factor: 5.103

5.  Retroviral DNA Transposition: Themes and Variations.

Authors:  Anna Marie Skalka
Journal:  Microbiol Spectr       Date:  2014-12

6.  Eukaryotic elongation factor 1 complex subunits are critical HIV-1 reverse transcription cofactors.

Authors:  Kylie Warren; Ting Wei; Dongsheng Li; Fangyun Qin; David Warrilow; Min-Hsuan Lin; Haran Sivakumaran; Ann Apolloni; Catherine M Abbott; Alun Jones; Jenny L Anderson; David Harrich
Journal:  Proc Natl Acad Sci U S A       Date:  2012-05-24       Impact factor: 11.205

7.  Endogenous reverse transcription of human immunodeficiency virus type 1 in physiological microenviroments: an important stage for viral infection of nondividing cells.

Authors:  H Zhang; G Dornadula; R J Pomerantz
Journal:  J Virol       Date:  1996-05       Impact factor: 5.103

8.  Mechanism of HIV-1 RNA dimerization in the central region of the genome and significance for viral evolution.

Authors:  Dorota Piekna-Przybylska; Gaurav Sharma; Robert A Bambara
Journal:  J Biol Chem       Date:  2013-07-09       Impact factor: 5.157

9.  Strand transfer and elongation of HIV-1 reverse transcription is facilitated by cell factors in vitro.

Authors:  David Warrilow; Kylie Warren; David Harrich
Journal:  PLoS One       Date:  2010-10-06       Impact factor: 3.240

10.  Cholesterol depletion of human immunodeficiency virus type 1 and simian immunodeficiency virus with beta-cyclodextrin inactivates and permeabilizes the virions: evidence for virion-associated lipid rafts.

Authors:  David R M Graham; Elena Chertova; Joanne M Hilburn; Larry O Arthur; James E K Hildreth
Journal:  J Virol       Date:  2003-08       Impact factor: 5.103
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