OBJECTIVE: Differential diagnosis of infections that cause similar diseases and may be active simultaneously in the same geographical areas is greatly needed. Dengue and yellow fever viruses (DENV and YFV) are transmitted by the same species of mosquito and both can cause haemorrhagic fever symptoms. These viruses are active mainly in regions where expensive and sophisticated technologies are not available. Our objective was to develop a simple, reliable and easy-to-perform method to detect and identify these viruses. METHODS: We slightly modified a generic RT-PCR able to detect the mentioned viruses and other members of this genus: specific primers for each one of these viruses were designed and included in the nested reaction instead of one of the generic ones. The reaction was optimized and viruses are amplified giving rise to bands of different sizes distinguishable in agarose gels. RESULTS: This test is able to detect and identify the four DENVs and YFV to a high level of sensitivity and specificity and can be used with clinical samples. This simple, reliable and easy-to-perform method able to detect and identify dengue 1-4 and YFV can be used in poor endemic countries.
OBJECTIVE: Differential diagnosis of infections that cause similar diseases and may be active simultaneously in the same geographical areas is greatly needed. Dengue and yellow fever viruses (DENV and YFV) are transmitted by the same species of mosquito and both can cause haemorrhagic fever symptoms. These viruses are active mainly in regions where expensive and sophisticated technologies are not available. Our objective was to develop a simple, reliable and easy-to-perform method to detect and identify these viruses. METHODS: We slightly modified a generic RT-PCR able to detect the mentioned viruses and other members of this genus: specific primers for each one of these viruses were designed and included in the nested reaction instead of one of the generic ones. The reaction was optimized and viruses are amplified giving rise to bands of different sizes distinguishable in agarose gels. RESULTS: This test is able to detect and identify the four DENVs and YFV to a high level of sensitivity and specificity and can be used with clinical samples. This simple, reliable and easy-to-perform method able to detect and identify dengue 1-4 and YFV can be used in poor endemic countries.
Authors: Marcio R T Nunes; Gustavo Palacios; Keley N B Nunes; Samir M M Casseb; Lívia C Martins; Juarez A S Quaresma; Nazir Savji; W Ian Lipkin; Pedro F C Vasconcelos Journal: J Virol Methods Date: 2011-03-17 Impact factor: 2.014
Authors: Cristina Domingo; Matthias Niedrig; Anette Teichmann; Marco Kaiser; Leonid Rumer; Richard G Jarman; Oliver Donoso-Mantke Journal: PLoS Negl Trop Dis Date: 2010-10-05
Authors: Raphaëlle Klitting; Carlo Fischer; Jan F Drexler; Ernest A Gould; David Roiz; Christophe Paupy; Xavier de Lamballerie Journal: Genes (Basel) Date: 2018-08-21 Impact factor: 4.096
Authors: Gilberto A Santiago; Edgardo Vergne; Yashira Quiles; Joan Cosme; Jesus Vazquez; Juan F Medina; Freddy Medina; Candimar Colón; Harold Margolis; Jorge L Muñoz-Jordán Journal: PLoS Negl Trop Dis Date: 2013-07-11