Literature DB >> 16929939

Spectroscopy-based quantitative fluorescence resonance energy transfer analysis.

Jie Zheng1.   

Abstract

The combination of green fluorescent protein mutants and fluorescence resonance energy transfer (FRET) forms a powerful tool for ion channel studies. A key to successful application of green fluorescent protein-based FRET is to reliably separate the FRET signal from various non-FRET fluorescence emissions that coexist in any experimental system. This chapter introduces a FRET quantification method that is based on fluorescence spectroscopic microscopy. Application of this "spectra FRET" method to both the confocal imaging of Xenopus oocytes and the epifluorescence imaging of culture cells is described. The fluorescence intensity ratio measurement, a complementary non-FRET method for identifying the channel subunit stoichiometry, is also discussed.

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Year:  2006        PMID： 16929939     DOI： 10.1385/1-59745-095-2:65

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  13 in total

1.  Application of fluorescence resonance energy transfer in protein studies.

Authors:  Linlin Ma; Fan Yang; Jie Zheng
Journal:  J Mol Struct       Date:  2014-11-05       Impact factor: 3.196

2.  Selective interaction of syntaxin 1A with KCNQ2: possible implications for specific modulation of presynaptic activity.

Authors:  Noa Regev; Nurit Degani-Katzav; Alon Korngreen; Adi Etzioni; Sivan Siloni; Alessandro Alaimo; Dodo Chikvashvili; Alvaro Villarroel; Bernard Attali; Ilana Lotan
Journal:  PLoS One       Date:  2009-08-13       Impact factor: 3.240

Review 3.  Fluorescence applications in molecular neurobiology.

Authors:  Justin W Taraska; William N Zagotta
Journal:  Neuron       Date:  2010-04-29       Impact factor: 17.173

4.  Localization of the PIP2 sensor of TRPV1 ion channels.

Authors:  Carmen A Ufret-Vincenty; Rebecca M Klein; Li Hua; Juan Angueyra; Sharona E Gordon
Journal:  J Biol Chem       Date:  2011-01-11       Impact factor: 5.157

5.  Thermosensitive TRPV channel subunits coassemble into heteromeric channels with intermediate conductance and gating properties.

Authors:  Wei Cheng; Fan Yang; Christina L Takanishi; Jie Zheng
Journal:  J Gen Physiol       Date:  2007-03       Impact factor: 4.086

6.  Competitive and non-competitive regulation of calcium-dependent inactivation in CaV1.2 L-type Ca2+ channels by calmodulin and Ca2+-binding protein 1.

Authors:  Shimrit Oz; Adva Benmocha; Yehezkel Sasson; Dana Sachyani; Lior Almagor; Amy Lee; Joel A Hirsch; Nathan Dascal
Journal:  J Biol Chem       Date:  2013-03-25       Impact factor: 5.157

7.  TRPV1 channels and the progesterone receptor Sig-1R interact to regulate pain.

Authors:  Miguel Ortíz-Rentería; Rebeca Juárez-Contreras; Ricardo González-Ramírez; León D Islas; Félix Sierra-Ramírez; Itzel Llorente; Sidney A Simon; Marcia Hiriart; Tamara Rosenbaum; Sara L Morales-Lázaro
Journal:  Proc Natl Acad Sci U S A       Date:  2018-01-29       Impact factor: 11.205

8.  Probing ion channel macromolecular interactions using fluorescence resonance energy transfer.

Authors:  Sharen Rivas; Khadija Hanif; Nourdine Chakouri; Manu Ben-Johny
Journal:  Methods Enzymol       Date:  2021-03-15       Impact factor: 1.600

Review 9.  Fluorescence-Based Strategies to Investigate the Structure and Dynamics of Aptamer-Ligand Complexes.

Authors:  Cibran Perez-Gonzalez; Daniel A Lafontaine; J Carlos Penedo
Journal:  Front Chem       Date:  2016-08-03       Impact factor: 5.221

10.  Interaction of Purinergic P2X4 and P2X7 Receptor Subunits.

Authors:  Markus Schneider; Kirsten Prudic; Anja Pippel; Manuela Klapperstück; Ursula Braam; Christa E Müller; Günther Schmalzing; Fritz Markwardt
Journal:  Front Pharmacol       Date:  2017-11-22       Impact factor: 5.810
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