Literature DB >> 1692715

Simplified northern blot hybridization using 5% sodium dodecyl sulfate.

G D Virca1, W Northemann, B R Shiels, G Widera, S Broome.   

Abstract

We describe a simplified Northern blot hybridization procedure. This procedure eliminates the need for many reagents commonly used in RNA hybridization and replaces them with two buffers containing 5% sodium dodecyl sulfate for pre-hybridization, hybridization and all post-hybridization washes.

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Year:  1990        PMID： 1692715

Source DB:  PubMed          Journal:  Biotechniques        ISSN： 0736-6205            Impact factor:   1.993


  40 in total

1.  The third subunit of protein phosphatase 2A (PP2A), a 55-kilodalton protein which is apparently substituted for by T antigens in complexes with the 36- and 63-kilodalton PP2A subunits, bears little resemblance to T antigens.

Authors:  D C Pallas; W Weller; S Jaspers; T B Miller; W S Lane; T M Roberts
Journal:  J Virol       Date:  1992-02       Impact factor: 5.103

2.  Characterization of circular mitochondrial plasmids in three Pythium species.

Authors:  F N Martin
Journal:  Curr Genet       Date:  1991-07       Impact factor: 3.886

3.  Respiratory syncytial virus-inducible BCL-3 expression antagonizes the STAT/IRF and NF-kappaB signaling pathways by inducing histone deacetylase 1 recruitment to the interleukin-8 promoter.

Authors:  Mohammad Jamaluddin; Sanjeev Choudhary; Shaofei Wang; Antonella Casola; Ruksana Huda; Roberto P Garofalo; Sutapa Ray; Allan R Brasier
Journal:  J Virol       Date:  2005-12       Impact factor: 5.103

4.  Cloning of RNA molecules in vitro.

Authors:  H V Chetverina; A B Chetverin
Journal:  Nucleic Acids Res       Date:  1993-05-25       Impact factor: 16.971

5.  Bacterial phospholipase C upregulates matrix metalloproteinase expression by cultured epithelial cells.

Authors:  J D Firth; E E Putnins; H Larjava; V J Uitto
Journal:  Infect Immun       Date:  1997-12       Impact factor: 3.441

6.  Adjacent and divergently oriented operons under the control of the sporulation regulatory protein GerE in Bacillus subtilis.

Authors:  S Roels; R Losick
Journal:  J Bacteriol       Date:  1995-11       Impact factor: 3.490

7.  Interferon-gamma inhibits macrophage apolipoprotein E production by posttranslational mechanisms.

Authors:  K Brand; N Mackman; L K Curtiss
Journal:  J Clin Invest       Date:  1993-05       Impact factor: 14.808

8.  Tolerance to endotoxin-induced expression of the interleukin-1 beta gene in blood neutrophils of humans with the sepsis syndrome.

Authors:  C E McCall; L M Grosso-Wilmoth; K LaRue; R N Guzman; S L Cousart
Journal:  J Clin Invest       Date:  1993-03       Impact factor: 14.808

9.  Evolutionary conservation of the AU-rich 3' untranslated region of messenger RNA.

Authors:  M A Asson-Batres; S L Spurgeon; J Diaz; T G DeLoughery; G C Bagby
Journal:  Proc Natl Acad Sci U S A       Date:  1994-02-15       Impact factor: 11.205

10.  Streptomyces coelicolor genes ftsL and divIC play a role in cell division but are dispensable for colony formation.

Authors:  Jennifer A Bennett; Rachel M Aimino; Joseph R McCormick
Journal:  J Bacteriol       Date:  2007-10-19       Impact factor: 3.490
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