Literature DB >> 1692714

Stability of RNA isolated from macrophages depends on the removal of an RNA-degrading activity early in the extraction procedure.

R Evans1, S J Kamdar.   

Abstract

Ubiquitous RNases are the usual causes of RNA degradation on its isolation from mammalian cells. Using guanidine hydrochloride for the extraction of RNA from mouse peritoneal macrophages, we identify a major source of RNA-degrading activity, the stage of the extraction procedure at which this activity may be detected and show that its removal early in the extraction leads to a more dependable method for the recovery of high quality RNA.

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Year:  1990        PMID： 1692714

Source DB:  PubMed          Journal:  Biotechniques        ISSN： 0736-6205            Impact factor:   1.993


  12 in total

1.  The transcriptional start site for a human U6 small nuclear RNA gene is dictated by a compound promoter element consisting of the PSE and the TATA box.

Authors:  R S Goomer; G R Kunkel
Journal:  Nucleic Acids Res       Date:  1992-09-25       Impact factor: 16.971

2.  Viral and host cellular transcription in Autographa californica nuclear polyhedrosis virus-infected gypsy moth cell lines.

Authors:  D Guzo; H Rathburn; K Guthrie; E Dougherty
Journal:  J Virol       Date:  1992-05       Impact factor: 5.103

3.  Interleukin-8 (IL-8) is a major neutrophil chemotaxin from human alveolar macrophages stimulated with staphylococcal enterotoxin A (SEA).

Authors:  E J Miller; S Nagao; F K Carr; J M Noble; A B Cohen
Journal:  Inflamm Res       Date:  1996-08       Impact factor: 4.575

4.  Fulminant hepatic failure in murine hepatitis virus strain 3 infection: tissue-specific expression of a novel fgl2 prothrombinase.

Authors:  J W Ding; Q Ning; M F Liu; A Lai; J Leibowitz; K M Peltekian; E H Cole; L S Fung; C Holloway; P A Marsden; H Yeger; M J Phillips; G A Levy
Journal:  J Virol       Date:  1997-12       Impact factor: 5.103

5.  Loss of resistance to murine hepatitis virus strain 3 infection after treatment with corticosteroids is associated with induction of macrophage procoagulant activity.

Authors:  R J Fingerote; M Abecassis; M J Phillips; Y S Rao; E H Cole; J Leibowitz; G A Levy
Journal:  J Virol       Date:  1996-07       Impact factor: 5.103

6.  Glucagon-like peptide-2 protects impaired intestinal mucosal barriers in obstructive jaundice rats.

Authors:  Jun Chen; Jia-Tian Dong; Xiao-Jing Li; Ye Gu; Zhi-Jian Cheng; Yuan-Kun Cai
Journal:  World J Gastroenterol       Date:  2015-01-14       Impact factor: 5.742

7.  Stabilized expression of mRNA is associated with mycobacterial resistance controlled by Nramp1.

Authors:  D H Brown; W P Lafuse; B S Zwilling
Journal:  Infect Immun       Date:  1997-02       Impact factor: 3.441

8.  A partial cDNA clone of trypomastigote decay-accelerating factor (T-DAF), a developmentally regulated complement inhibitor of Trypanosoma cruzi, has genetic and functional similarities to the human complement inhibitor DAF.

Authors:  D V Tambourgi; T L Kipnis; W D da Silva; K A Joiner; A Sher; S Heath; B F Hall; G B Ogden
Journal:  Infect Immun       Date:  1993-09       Impact factor: 3.441

9.  Resistance to murine hepatitis virus strain 3 is dependent on production of nitric oxide.

Authors:  M Pope; P A Marsden; E Cole; S Sloan; L S Fung; Q Ning; J W Ding; J L Leibowitz; M J Phillips; G A Levy
Journal:  J Virol       Date:  1998-09       Impact factor: 5.103

10.  Association of mouse fibrinogen-like protein with murine hepatitis virus-induced prothrombinase activity.

Authors:  R L Parr; L Fung; J Reneker; N Myers-Mason; J L Leibowitz; G Levy
Journal:  J Virol       Date:  1995-08       Impact factor: 5.103
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