Literature DB >> 16926515

A rapid method for protein extraction from fission yeast.

Yuzy Matsuo1, Kazuhide Asakawa, Takashi Toda, Satoshi Katayama.   

Abstract

Researchers working with fission yeast conduct protein extraction widely and frequently, but this includes the handling of glass beads, and hence is laborious and cumbersome, especially when dealing with a large number of samples. Here we describe a rapid and reliable method for preparing protein extract from fission yeast, one which is applicable to routine western blotting.

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Year:  2006        PMID： 16926515     DOI： 10.1271/bbb.60087

Source DB:  PubMed          Journal:  Biosci Biotechnol Biochem        ISSN： 0916-8451            Impact factor:   2.043


  31 in total

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5.  Multistep regulation of protein kinase A in its localization, phosphorylation and binding with a regulatory subunit in fission yeast.

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9.  Expression profiling of S. pombe acetyltransferase mutants identifies redundant pathways of gene regulation.

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10.  The human Holliday junction resolvase GEN1 rescues the meiotic phenotype of a Schizosaccharomyces pombe mus81 mutant.

Authors:  Alexander Lorenz; Stephen C West; Matthew C Whitby
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