Advanced NMR approaches for a detailed structure analysis of natural products.

Some new nuclear magnetic resonance (NMR) approaches to elucidate chemical structures, which have not been determined by routine NMR methods, are presented. Selective detection of methine (CH), methylene (CH(2)), or methyl (CH(3)) signals in each subspectrum by editing NMR methods was utilized to reduce the complexity in crowded spectra. It also increased the peak separation and enhanced the sensitivity by limiting the measuring area of the 2D spectra. Several 2D methods to measure (2,3)J(CH) values, which are useful for stereochemical assignment are then introduced. To determine the structure of a highly hydrogen-deficient molecule, efficient correlation methods for long-range (13)C-(13)C coupling and (1)H-(15)N HMBC are also described.